A new ganglioside showing choleragenoid-binding activity in mouse spleen.
A new ganglioside showing choleragenoid-binding activity in mouse spleen.
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一种新的神经节苷脂在小鼠脾脏中显示出类胆原素结合活性。
DOI：
10.1093/oxfordjournals.jbchem.a121949
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发表时间：
1987
影响因子：
2.7
通讯作者：
A. Suzuki
中科院分区：
文献类型：
--
作者：
K. Nakamura;M. Suzuki;F. Inagaki;T. Yamakawa;A. Suzuki
从小鼠脾脏中分离纯化了一种新的具有类胆固醇结合活性的神经节苷脂。根据糖组成分析、酶水解、全甲基化研究、1H-NMR光谱和负离子快原子轰击质谱的结果，神经节苷脂的结构确定如下：Gal beta 1-3GalNAc beta 1-4Gal beta 1-3GalNAc beta 1-4Gal beta 1 -4Glc beta 1- 1 '神经酰胺3-NeuGc α 2该神经节苷脂含有与II 3 NeuGc α-Gg 4 Cer（GM 1（NeuGc））相同的末端四糖结构。通过TLC-免疫结合试验和酶联免疫吸附试验，神经节苷脂被证明具有几乎相同的类胆固醇结合活性的GM 1。另一种神经节苷脂，比新的类霍乱毒素结合神经节苷脂迁移更快，也从相同的源材料中纯化，并鉴定为IV 4GalNAc β，IV 3 NeuGc α-Gg 4Cer（GalNAc-GM 1b（NeuGc））。因为，在先前的研究中，我们证明了小鼠脾脏中IV 3 NeuGc α-Gg 4 Cer（GM 1b（NeuGc））的存在（中村，K.等人（1984）J.Biochem.96，949-957），该研究的结果表明，新的类霍乱原结合神经节苷脂是从GM 1b（NeuGc）通过GalNAc-GM 1b（NeuGc）合成的。
A new ganglioside showing choleragenoid-binding activity was purified from mouse spleen and characterized. From the results of sugar-composition analysis, enzymatic hydrolysis, a permethylation study, 1H-NMR spectroscopy, and negative-ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows: Gal beta 1-3GalNAc beta 1-4Gal beta 1-3GalNAc beta 1-4Gal beta 1-4Glc beta 1-1'ceramide 3----NeuGc alpha 2 This ganglioside contains a terminal tetrasaccharide structure identical with that of II3NeuGc alpha-Gg4Cer (GM1(NeuGc]. By means of a TLC-immunobinding assay and an enzyme-linked immunosorbent assay, the ganglioside was demonstrated to have almost the same choleragenoid-binding activity as GM1. Another ganglioside, that migrated faster than the new choleragenoid-binding ganglioside, was also purified from the same source material and identified as IV4GalNAc beta,IV3NeuGc alpha-Gg4Cer (GalNAc-GM1b(NeuGc]. Since, in the previous study, we demonstrated the existence of IV3NeuGc alpha-Gg4Cer (GM1b(NeuGc] in mouse spleen (Nakamura, K. et al. (1984) J. Biochem. 96, 949-957), the results of this study suggest that the new choleragenoid-binding ganglioside is synthesized from GM1b(NeuGc) through GalNAc-GM1b(NeuGc).