MicroRNA-dependent regulation of metamorphosis and identification of microRNAs in the red flour beetle, Tribolium castaneum
MicroRNA-dependent regulation of metamorphosis and identification of microRNAs in the red flour beetle, Tribolium castaneum
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赤拟谷盗变态的 MicroRNA 依赖性调节和 microRNA 的鉴定
DOI：
10.1016/j.ygeno.2017.06.001
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发表时间：
2017
期刊：
影响因子：
4.4
通讯作者：
Li Bin
中科院分区：
文献类型：
--
作者：
Wu Wei;Xiong Wenfeng;Li Chengjun;Zhai Mengfan;Li Yao;Ma Fei;Li Bin
到目前为止，虽然已经在全代谢昆虫--卡氏赤霉菌中发现了一些microRNAs(MiRNAs)，但仍有大量的miRNAs需要研究。在晚期幼虫中，敲除Dager-1(Dcr-1)和ArgAerte-1(AGO-1)基因会损害miRNA的合成，并通过上调耐甲基丁二烯(Met)和Krüppel-H1mRNA水平影响保幼激素途径，导致一系列缺陷。铸件的发育和变态。因此，高通量的Illumina/Solexa测序是用八个关键发育阶段的混合样本进行的。卡加尼姆。总共发现了1154个独特的miRNAs，包括来自68个miRNA家族的274个保守的miRNAs，108个已知的候选miRNAs和772个新的miRNAs。基因组位置分析表明，miRNA簇的int含量更丰富。与其他物种相比，它更像是一种。结果表明，Dcr-1和Ago-1在T.蓖麻素导致miRNA诱导的变态缺陷。此外，还发现了大量新的miRNAs。卡斯特兰和本地化的TOT。卡斯塔基因组基因座。
To date, although some microRNAs (miRNAs) have been discovered in the holometabolism insectTribolium castaneum, large numbers of miRNAs still require investigation. Knocking downDicer-1(Dcr-1) andArgonaute-1(Ago-1) in late larvae impaired miRNA synthesis, affected the juvenile hormone pathway by up-regulatingMethoprene-tolerant(Met) andKrüppel-homolog1(Kr-h1) transcript levels, and resulted in a series of defects inT. castaneumdevelopment and metamorphosis. Thus, high-throughput Illumina/Solexa sequencing was performed with a mixed sample of eight key developmental stages ofT. castaneum. In total, 1154 unique miRNAs were discovered containing 274 conserved miRNAs belong to 68 miRNA families, 108 known candidate miRNAs and 772 novel miRNAs. Genome locus analysis showed that miRNA clusters are more abundant inT. castaneumthan other species. The results indicated that RNAi ofDcr-1andAgo-1inT. castaneumresulted in miRNA-induced metamorphosis defects. Furthermore, large numbers of novel miRNAs were discovered inT. castaneumand localized toT. castaneumgenome loci.