ISOLATION OF INSECT PATHOGENIC BACTERIA, PROVIDENCIA-RETTGERI, FROM HETERORHABDITIS SPP
ISOLATION OF INSECT PATHOGENIC BACTERIA, PROVIDENCIA-RETTGERI, FROM HETERORHABDITIS SPP
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DOI：
10.1111/j.1365-2672.1995.tb05022.x
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发表时间：
1995-03-01
期刊：
影响因子：
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通讯作者：
DOWDS, BCA
中科院分区：
文献类型：
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作者：
JACKSON, TJ;WANG, HY;DOWDS, BCA
异杆线虫属的线虫携带光杆菌属的细菌进入昆虫，包括园艺作物的害虫。细菌杀死昆虫，并为线虫的生长和发展提供条件。据报道，异尖线虫的主要种类是异尖线虫。测试的菌株含有第二种细菌，被鉴定为普罗维登西亚雷特吉利。将该细菌注入蜡蛾幼虫体内，结果表明，雷氏拟青霉的致病性至少与Phtorhabdus sp.相当。K122路。两者的LD(50)值都不到一个细菌细胞/幼虫，但在28℃和9℃下，雷氏拟青霉对昆虫的杀灭速度都比K122快得多。由于在低温下杀虫速度很慢，因此在这种条件下，雷氏拟青霉可能是一种更好的害虫防治剂。然而，由线虫携带到昆虫幼虫体内的P.rettgeri是不致病的，表明线虫抑制了其释放或致病性。为了发挥其在害虫防治方面的潜力，有必要找到绕过或抑制这种抑制的方法。
Nematodes of the genus Heterorhabditis carry bacteria of the genus Photorhabdus into insects including pests of horticultural crops. The bacteria kill the insect and provide conditions which allow for the growth and development of the nematodes. It is reported here that the majority of Heterorhabditis spp. strains tested contained a second bacterial species which was identified as Providencia rettgeri. Injection of the bacteria into waxmoth larvae showed that P. rettgeri was at least as pathogenic as Photorhabdus sp. K122. Both had LD(50) values of less than one bacterial cell/larva, but P. rettgeri killed the insects at a considerably faster rate than K122 at both 28 degrees C and 9 degrees C. Since Photorhabdus kills very slowly at low temperatures, it appeared that P. rettgeri might be a better pest control agent under these conditions. However, P. rettgeri was not pathogenic when carried into insect larvae by the nematode, indicating that the nematode suppressed either its release or pathogenicity. It will be necessary to find ways of bypassing or inhibiting this suppression for P. rettgeri to fulfil its potential in pest control.