NONADRENAL EPINEPHRINE-FORMING ENZYMES IN HUMANS - CHARACTERISTICS, DISTRIBUTION, REGULATION, AND RELATIONSHIP TO EPINEPHRINE LEVELS
NONADRENAL EPINEPHRINE-FORMING ENZYMES IN HUMANS - CHARACTERISTICS, DISTRIBUTION, REGULATION, AND RELATIONSHIP TO EPINEPHRINE LEVELS
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DOI：
10.1172/jci117996
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发表时间：
1995-06-01
影响因子：
15.9
通讯作者：
ZIEGLER, MG
中科院分区：
文献类型：
--
作者：
KENNEDY, B;BIGBY, TD;ZIEGLER, MG
动物实验表明，非肾上腺组织也能合成肾上腺素（E）。本文研究了人肺、肾、心、肝、脾和胰腺中的苯乙醇胺N-甲基转移酶（PNMT）和/或非特异性N-甲基转移酶（NMT）的活性。（r = 0.34），人体组织中的PNMT和NMT在底物和抑制剂特异性、热稳定性和抗原性方面不同。人肺和人支气管上皮细胞中的PNMT与肾上腺PNMT无法区分。PNMT和/或NMT活性存在于红细胞（RBC）和癌细胞系中。人肾、肺和胰腺显示肾上腺PNMT抗体的免疫组织化学染色，RBC PNMT活性在男性中低于女性，并且在甲状腺功能亢进症中增加，在甲状腺功能减退症中减少，人支气管上皮细胞系中的PNMT活性通过与地塞米松孵育而显著增加，静脉滴注H-3-E时血浆和尿液中E和H-3-E的含量表明，肾脏可合成尿中一半的E。我们认为PNMT和NMT广泛分布于人体各组织中，它们可在体内合成E，并受糖皮质激素和甲状腺激素的影响。
Animal studies indicate that nonadrenal tissues may synthesize epinephrine (E), Here we demonstrate phenylethanolamine N-methyltransferase (PNMT) and/or nonspecific N-methyltransferase (NMT) enzymatic activity in human lung, kidney, heart, liver, spleen, and pancreas, There was a significant overall correlation (r = 0.34) between tissue PNMT and E, PNMT and NMT in human tissues differed in substrate and inhibitor specificity, thermal stability, and antigenicity, By these criteria, PNMT in human lung and in human bronchial epithelial cells were indistinguishable from adrenal PNMT. PNMT and/or NMT activity were present in red blood cells (RBCs), and cancer cell lines, Human kidney, lung, and pancreas showed immunohistochemical staining with an antibody to adrenal PNMT, RBC PNMT activity was lower in males than females and was increased in hyperthyroidism and decreased in hypothyroidism, PNMT activity in a human bronchial epithelial cell line was dramatically increased by incubation with dexamethasone, E and H-3-E levels in plasma and urine during an intravenous infusion of H-3-E into humans indicated ghat kidney may synthesize half of urinary E, We conclude that PNMT and NMT are widely distributed in human tissues, that they may synthesize E in vivo and are influenced by glucocorticoid and thyroid hormones.