Assessment of major histocompatibility complex class I interaction with Epstein‐Barr virus and human immunodeficiency virus peptides by elevation of membrane H‐2 and HLA in peptide loading‐deficient cells
Assessment of major histocompatibility complex class I interaction with Epstein‐Barr virus and human immunodeficiency virus peptides by elevation of membrane H‐2 and HLA in peptide loading‐deficient cells
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通过肽负载缺陷细胞中膜 H-2 和 HLA 的升高来评估主要组织相容性复合体 I 类与 Epstein-Barr 病毒和人类免疫缺陷病毒肽的相互作用
DOI：
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发表时间：
1992
影响因子：
5.4
通讯作者：
G. Klein
中科院分区：
文献类型：
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作者：
G. Stuber;S. Modrow;P. Höglund;L. Franksson;J. Elvin;H. Wolf;K. Kärre;G. Klein
早期的研究结果表明，肽可以影响具有缺陷肽加载机制的细胞表面上的主要组织相容性复合物（MHC）I类分子的表达。我们已经使用肽诱导的I类抗原表达的增加来评估肽与MHC I类分子的相互作用。评估了一组41种来自人免疫缺陷病毒-1（HIV-1）gag蛋白的重叠合成肽和33种来自爱泼斯坦-巴尔病毒（EBV）蛋白EBNA-1、2、3、4、5、6、LMP、BZLF 2、BILF 2、BSLF 2、BALF 4和BcLF 1的非重叠肽增强HLA-A2. 1、H-2Db、通过间接免疫荧光测定鼠RMA‐S和人721.174/T2（.174/T2）细胞系上的Kb和Dd。考虑到肽处理样品中荧光强度加倍为阳性，发现39种HIV中的6种和32种EBV中的1种与A2.1结合，39种HIV gag中的6种和16种EBV中的7种与Db结合，39种HIV gag中的8种和16种EBV中的5种与Kb结合，39种HIV gag中的2种和17种EBV中的1种与Dd结合。该方法的灵敏度与使用构象依赖性单克隆抗体的体外I类组装试验相当，并且比固相试验更具鉴别力。由于其简单性，该方法也可用于测试大肽组与各种I类分子的结合能力。此外，该方法提供了有关活细胞中I类组装体体外试验相关性的信息。
Earlier findings indicate that peptides can affect the expression of major histocompatibility complex (MHC) class I molecules on the surface of cells with defective peptide loading mechanism. We have used peptide induced increase of class I antigen expression to assess peptide interaction with MHC class I molecules. A panel of 41 overlapping synthetic peptides derived from the human immunodeficiency virus‐1 (HIV‐1) gag protein and 33 nonoverlapping peptides from Epstein‐Barr virus (EBV) proteins EBNA‐1, 2, 3, 4, 5, 6, LMP, BZLF2, BILF2, BSLF2, BALF4 and BcLF1 was assessed for the ability to enhance the expression of HLA‐A2.1, H‐2Db, Kb and Dd on the murine RMA‐S and human 721.174/T2 (.174/T2) lines by indirect immunofluorescence. Considering doubling of the fluorescence intensity in the peptide‐treated samples as positivity, 6 of 39 HIV and 1 of 32 EBV peptides were found to bind to A2.1, 6 of 39 HIV gag and 7 of 16 EBV peptides to Db, 8 of 39 HIV gag and 5 of 16 EBV peptides to Kb and 2 of 39 HIV gag and 1 of 17 EBV peptides to Dd. The sensitivity of the method is comparable to the in vitro class I assembly assay with conformation‐dependent monoclonal antibody and is more discriminating than the solid‐phase assay. Due to its simplicity this method can also serve for testing large peptide panels for binding capacity to various class I molecules. Moreover, the method provides information about the relevance of in vitro tests for class I assembly in living cells.
淋巴瘤突变体中对 H-2 限制性但对同种异体 H2 特异性移植物和细胞毒性 T 淋巴细胞反应没有抵抗力。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Ohlen,C;Bastin,J;Ljunggren,HG;Foster,L;Wolpert,E;Klein,G;Townsend,AR;Karre,K
通讯作者：
Karre,K
DOI：
10.1073/pnas.82.15.5131
发表时间：
1985-01-01
影响因子：
11.1
作者：
HOUGHTEN, RA
通讯作者：
HOUGHTEN, RA
影响因子：
56.9
作者：
HENDERSON, RA;MICHEL, H;ENGELHARD, VH
通讯作者：
ENGELHARD, VH