The effect of low levels of lead (Pb) in the blood on levels of sphingosine-1-phosphate (S1P) and expression of S1P receptor 1 in the brain of the rat in the perinatal period
The effect of low levels of lead (Pb) in the blood on levels of sphingosine-1-phosphate (S1P) and expression of S1P receptor 1 in the brain of the rat in the perinatal period
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DOI：
10.1016/j.chemosphere.2016.09.067
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发表时间：
2017-01-01
期刊：
影响因子：
8.8
通讯作者：
Gutowska, I.
中科院分区：
文献类型：
--
作者：
Lukomska, A.;Baranowska-Bosiacka, I.;Gutowska, I.
鞘脂是脂质膜的主要成分。它们还执行结构功能，并参与许多信号传输过程。鞘氨醇-1-磷酸（S1 P）是一种具有生物活性的鞘脂，它是五种G蛋白偶联受体（S1 PRs 1 -5）的配体，也可以作为细胞内的第二信使。S1 P是负责刺激祖细胞在大脑中，但它也可以诱导成熟neurons.This研究的目的是评估的影响，前和新生儿暴露于允许的铅浓度对SW水平和S1 PR 1（EDG 1）表达在前额叶皮层，小脑和海马的大鼠。采用RP-HPLC法测定S1 P的浓度，RT PCR和Western Blot法测定S1 PR 1的表达，免疫组化法测定S1 PR 1受体的免疫定位。我们的研究结果表明，即使是低血铅浓度，即在可接受的限度内的10微克/分升引起的变化，在小脑，前额皮质和海马体中的S1 P的浓度。我们的数据还显示，在大脑的所有研究部分中，S1 PR 1的水平显著降低，而S1 PR 1基因表达没有显著变化。前和新生儿暴露于铅也导致S1 PR 1在神经胶质细胞中的表达减少在所有地区的角Ammonis（CM-CA 4）和齿状回在海马，以及在所有层的小脑和前额皮质，与未暴露的对照组相比。(C)2016爱思唯尔有限公司版权所有
Sphingolipids are the main components of the lipid membrane. They also perform structural functions and participate in many signal transmission processes. One of the bioactive sphingolipids is sphingosine-1-phosphate (S1P), a ligand for five G protein-coupled receptors (S1PRs1-5), which can also act as an intracellular second messenger. S1P is responsible for the stimulation of progenitor cells in the brain, but it can also induce apoptosis of mature neurons.This study is aimed at assessing the effect of pre- and neonatal exposure to permissible Pb concentrations on SW levels and S1PR1 (EDG1) expression in the prefrontal cortex, cerebellum, and hippo campus of rats. The concentrations of S1P were determined by RP-HPLC, S1PR1 expression was determined by RT PCR and Western Blot, and receptor immunolocalization was determined by immunohistochemistry method. Our results showed that even low blood Pb concentrations, i.e. within the acceptable limit of 10 mu g/dL caused changes in the concentration of S1P in the cerebellum, prefrontal cortex, and hippocampus. Our data also showed a significant decrease in the level of S1PR1 in all studied part of brain, without significant changes in S1PR1 gene expression. Pre- and neonatal exposure to Pb also resulted in a decrease in the expression of S1PR1 in glial cells in all regions of the Cornu Ammonis (CM-CA4) and Dentate Gyrus in the hippocampus, as well as in all layers of the cerebellum and prefrontal cortex, compared to the unexposed control group. (C) 2016 Elsevier Ltd. All rights reserved.