Immunochemical assessment of deoxynivalenol tissue distribution following oral exposure in the mouse
Immunochemical assessment of deoxynivalenol tissue distribution following oral exposure in the mouse
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DOI：
10.1016/j.toxlet.2008.02.005
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发表时间：
2008-05-05
影响因子：
3.5
通讯作者：
Amuzie, Chidozie J.
中科院分区：
文献类型：
--
作者：
Pestka, James J.;Islam, Zahidul;Amuzie, Chidozie J.
脱氧雪腐镰刀菌烯醇（DON或呕吐毒素）是一种常见于谷物中的腐镰刀菌烯真菌毒素，对实验动物的生长和免疫功能产生不利影响。采用竞争性酶联免疫吸附试验（ELISA）监测口服25 mg/kg bw毒素的年轻成年B6 C3 F1雄性小鼠组织中DON的分布和清除动力学。DON在血浆、肝、脾和脑中的检测时间为5 min ~ 24 h，在心和肾中的检测时间为5 min ~ 8 h。在给药后5-15分钟内观察到最高DON血浆浓度（12 μ g/mL）。两室动力学（t（1/2）α = 20.4 min，t（1/2）β = 11.8 h）后，DON的清除率迅速，8 h和24 h后，DON的最大血浆浓度分别为5%和2%。DON在其他组织中的分布和清除动力学与血浆相似。在5分钟时，以μ g/g计的DON浓度在肝脏中为19.5 +/- 1.9，在肾脏中为7.6 +/- 0.5，在脾脏中为7.3 +/- 0.8，在心脏中为6.8 +/- 0.9，在脑中为0.8 +/- 0.1。通过ELISA测定的组织中DON回收率与采用1H-DON和25 mg/kg bw DON剂量的先前研究相当。该方法可用于经饲料染毒小鼠血浆中DON的检测。这种方法提供了一种简单的策略，可用于回答啮齿动物中剂量、物种、年龄、性别、遗传背景和暴露途径/持续时间如何影响DON摄取和清除的相关问题。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Deoxynivalenol (DON or vomitoxin) is a trichothecene mycotoxin commonly found in cereal grains that adversely affects growth and immune function in experimental animals. A competitive enzyme-linked immunosorbent assay (ELISA) was used to monitor the kinetics of distribution and clearance of DON in tissues of young adult B6C3F1 male mice that were orally administered 25 mg/kg bw of the toxin. DON was detectable from 5 min to 24 h in plasma, liver, spleen and brain and from 5 min to 8 h in heart and kidney. The highest DON plasma concentrations were observed within 5-15 min (12 mu g/mL) after dosing. There was rapid clearance following two-compartment kinetics (t(1/2)alpha = 20.4 min, t(1/2)beta = 11.8 h) with 5% and 2% maximum plasma DON concentrations remaining after 8 and 24 h, respectively. DON distribution and clearance kinetics in other tissues were similar to that of plasma. At 5 min, DON concentrations in mu g/g were 19.5 +/- 1.9 in liver, 7.6 +/- 0.5 in kidney, 7.3 +/- 0.8 in spleen, 6.8 +/- 0.9 in heart and 0.8 +/- 0.1 in the brain. DON recoveries in tissues by ELISA were comparable to a previous study that employed I H-DON and 25 mg/kg bw DON dose. The ELISA was further applicable to the detection of DON in plasma of mice exposed to the toxin via diet. This approach provides a simple strategy that can be used to answer relevant questions in rodents of how dose, species, age, gender, genetic background and route/duration of exposure impact DON uptake and clearance. (C) 2008 Elsevier Ireland Ltd. All rights reserved.