HSP10 selective preference for myeloid and megakaryocytic precursors in normal human bone marrow.
HSP10 selective preference for myeloid and megakaryocytic precursors in normal human bone marrow.
复制标题
DOI：
10.4081/895
复制
发表时间：
2004-07
期刊：
影响因子：
--
通讯作者：
F. Cappello;C. Tripodo;F. Farina;V. Franco;G. Zummo
中科院分区：
文献类型：
--
作者：
F. Cappello;C. Tripodo;F. Farina;V. Franco;G. Zummo
热休克蛋白（HSP）构成了参与细胞稳态的异质蛋白家族。在细胞生命过程中，它们参与有害的损伤以及免疫和炎症反应。众所周知，它们调节基因表达以及细胞增殖、分化和死亡。 HSP60 是一种线粒体伴侣蛋白，在进化过程中高度保留，负责蛋白质折叠。其功能在原核和真核元件中都严格依赖于HSP10。我们通过免疫组织化学方法研究了一系列 20 份正常人骨髓标本 (NHBM) 中 HSP60 和 HSP10 的存在和表达。 NHBM 未显示 HSP60 表达，可能是因为其低于可检测阈值，这一点已在其他正常人体组织中得到证实。相比之下，HSP10 对骨髓细胞和巨核细胞谱系表现出选择性阳性。阳性仅限于前体细胞，而成熟元件始终呈阴性。我们假设 HSP10 除了作为线粒体辅伴侣之外，还在骨髓细胞分化中发挥作用。目前的数据强调了 HSP10 在细胞稳态中的作用，并鼓励该领域的进一步研究。
Heat shock proteins (HSPs) constitute a heterogeneous family of proteins involved in cell homeostasis. During cell life they are involved in harmful insults, as well as in immune and inflammatory reactions. It is known that they regulate gene expression, and cell proliferation, differentiation and death. HSP60 is a mitochondrial chaperonin, highly preserved during evolution, responsible of protein folding. Its function is strictly dependent on HSP10 in both prokaryotic and eukaryotic elements. We investigated the presence and the expression of HSP60 and HSP10 in a series of 20 normal human bone marrow specimens (NHBM) by the means of immunohistochemistry. NHBM showed no expression of HSP60, probably due to its being below the detectable threshold, as already demonstrated in other normal human tissues. By contrast, HSP10 showed a selective positivity for myeloid and megakaryocytic lineages. The positivity was restricted to precursor cells, while mature elements were constantly negative. We postulate that HSP10 plays a role in bone marrow cell differentiation other than being a mitochondrial co-chaperonin. The present data emphasize the role of HSP10 during cellular homeostasis and encourage further investigations in this field.