Serum protein changes in a rat model of chronic pain show a correlation between animal and humans.
Serum protein changes in a rat model of chronic pain show a correlation between animal and humans.
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DOI：
10.1038/srep41723
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发表时间：
2017-02-01
影响因子：
4.6
通讯作者：
Pini LA
中科院分区：
文献类型：
--
作者：
Bellei E;Vilella A;Monari E;Bergamini S;Tomasi A;Cuoghi A;Guerzoni S;Manca L;Zoli M;Pini LA
在以前的工作中，我们显示了一些蛋白质在慢性疼痛患者的生物液体中的过度表达。在这项蛋白质组学研究中，我们分析了血清从大鼠模型的神经性疼痛获得的慢性压迫性损伤（CCI）的坐骨神经，在两个时间间隔，2和5周后的侮辱，找到蛋白质参与表达或调解的疼痛。假手术和CCI大鼠用生理盐水或吲哚美辛治疗。结扎后两周，我们鉴定了CCI大鼠中过表达的三种血清蛋白，其中两种，α-1-巨球蛋白和维生素D结合蛋白（VDBP），在手术后5周仍然增加;在这个时间间隔，我们发现其他蛋白质的水平增加，即载脂蛋白A-I（APOA 1）、载脂蛋白E（APOE）、胰高血糖素-H2 D-异构酶（PTGDS）和甲状腺素运载蛋白（TTR），与人类中过度表达的蛋白质重叠。吲哚美辛治疗逆转结扎的影响。qPCR分析显示，术后5周用生理盐水处理的CCI大鼠的APOA 1、APOE、PTGDS和VDBP的转录水平在腰髓（坐骨神经的起源）中过表达，但在纹状体（不相关的脑区域）中未过表达，表明腰髓是这些蛋白质的可能来源。
In previous works we showed the overexpression of some proteins in biological fluids from patients suffering chronic pain. In this proteomic study we analysed serum from a rat model of neuropathic pain obtained by the chronic constriction injury (CCI) of sciatic nerve, at two time intervals, 2 and 5 weeks after the insult, to find proteins involved in the expression or mediation of pain. Sham-operated and CCI rats were treated with saline or indomethacin. Two weeks after ligation, we identified three serum proteins overexpressed in CCI rats, two of which, alpha-1-macroglobulin and vitamin D-binding protein (VDBP), remained increased 5 weeks post-surgery; at this time interval, we found increased levels of further proteins, namely apolipoprotein A-I (APOA1), apolipoprotein E (APOE), prostaglandin-H2 D-isomerase (PTGDS) and transthyretin (TTR), that overlap the overexpressed proteins found in humans. Indomethacin treatment reversed the effects of ligation. The qPCR analysis showed that transcript levels of APOA1, APOE, PTGDS and VDBP were overexpressed in the lumbar spinal cord (origin of sciatic nerve), but not in the striatum (an unrelated brain region), of CCI rats treated with saline 5 weeks after surgery, demonstrating that the lumbar spinal cord is a possible source of these proteins.