A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome.
A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome.
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发表时间：
1994-11
期刊：
影响因子：
3.3
通讯作者：
A. Reinisch;Jianghan Dong;C. Brubaker;D. Stelly;J. Wendel;A. Paterson
中科院分区：
文献类型：
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作者：
A. Reinisch;Jianghan Dong;C. Brubaker;D. Stelly;J. Wendel;A. Paterson
我们利用一个详细的限制性片段长度多态(RFLP)图谱来研究棉花这一二体多倍体的染色体组织和进化。约46.2%的核DNA探针检测到区分陆地棉和海岛棉的RFLP，705个RFLP基因座被聚为41个连锁群和4675 cM。显示了大多数连锁群的亚基因组起源(A与D)，以及14个(26个)连锁群的染色体同一性。A和D亚基因组显示相似的重组长度，这表明在物理上较大的A亚基因组中的重复DNA在重组上是惰性的。RFLP在D亚基因组中更为丰富。复制的RFLP之间的连接揭示了11对同源染色体区域--两个看起来是同序列的，大多数因倒位而不同，至少有一个因易位而不同。大多数同源涉及来自不同亚基因组的染色体，推测反映了1.1-190万年前n=13到n=26的多倍化事件。一些观察表明，另一个更早的多倍化事件产生了n=13棉花，至少在2500万年前。棉花基因组每厘米含有约400kb的DNA，因此基于图谱的基因克隆是可行的。棉花图谱为研究染色体进化和开发棉花遗传资源以改良世界领先的天然纤维提供了新的机会。
We employ a detailed restriction fragment length polymorphism (RFLP) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. About 46.2% of nuclear DNA probes detect RFLPs distinguishing Gossypium hirsutum and Gossypium barbadense; and 705 RFLP loci are assembled into 41 linkage groups and 4675 cM. The subgenomic origin (A vs. D) of most, and chromosomal identity of 14 (of 26), linkage groups is shown. The A and D subgenomes show similar recombinational length, suggesting that repetitive DNA in the physically larger A subgenome is recombinationally inert. RFLPs are somewhat more abundant in the D subgenome. Linkage among duplicated RFLPs reveals 11 pairs of homoelogous chromosomal regions-two appear homosequential, most differ by inversions, and at least one differs by a translocation. Most homoeologies involve chromosomes from different subgenomes, putatively reflecting the n = 13 to n = 26 polyploidization event of 1.1-1.9 million years ago. Several observations suggest that another, earlier, polyploidization event spawned n = 13 cottons, at least 25 million years ago. The cotton genome contains about 400-kb DNA per cM, hence map-based gene cloning is feasible. The cotton map affords new opportunities to study chromosome evolution, and to exploit Gossypium genetic resources for improvement of the world's leading natural fiber.