Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates
Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates
复制标题
通过与微孔板高度兼容的流体操纵装置捕获细胞聚集体
DOI：
10.1007/s10544-015-9953-x
复制
发表时间：
2015
影响因子：
2.8
通讯作者：
Shuhei Tajima and Yasuhiko Tabata
中科院分区：
文献类型：
--
作者：
Satoshi Konishi;Yumi Teramachi;Shuhei Shimomura;Wataru Tonomura;Shuhei Tajima and Yasuhiko Tabata
本文提出了一种捕获装置来操纵和运输微孔中的细胞聚集体。细胞聚集体（直径几百μm）目前通过移液器操作。通过吸移管的手动操作存在问题;低可靠性、低通量和难以确认任务完成。我们考虑到与现有方法如微孔板的兼容性，并设计用于微孔中细胞聚集体的捕获装置。新开发的捕获装置使细胞聚集体从孔的底部流动并携带到捕获装置的捕集器。我们在捕获装置的底部设计了一个曲面，以形成一个空间，作为微孔内壁之间的通道。本文介绍了概念，设计，制造，和建议的细胞聚集体捕获，成功的实验结果。
This paper proposes a capture device to manipulate and transport a cellular aggregate in a micro-well. A cellular aggregate (a few hundreds μm in diameter) is currently manipulated by a pipette. The manual manipulation by a pipette has problems; low reliability, low throughput, and difficulty in confirmation of task completion. We took into account of compatibility with existing methods such as a micro-well-plate and designed for the capture device of a cellular aggregate in a micro-well. A newly developed capture device flows and carries a cellular aggregate from a bottom of a well to a trap of the capture device. We designed a curved surface at the bottom of the capture device to form a space to act as a channel between the inner wall of the micro-well. This paper presents concept, design, fabrication, and of the proposed cellular aggregate capture, followed by successful experimental results.