Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window
Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window
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DOI：
10.1590/s0102-865020180110000008
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发表时间：
2018-11-01
期刊：
影响因子：
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通讯作者：
Campagnol, Daniela
中科院分区：
文献类型：
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作者：
Monteiro, Betânia Souza;Santos, Bianka Souza dos;Campagnol, Daniela
目的：方法：选用16周龄Wistar大鼠72只，行剖腹手术，夹闭肾动脉和肾静脉24 h。将动物随机分配到10个实验均质组，对应于磷酸盐缓冲盐水（PBS）或脂肪组织来源的间充质干细胞（ADSC）治疗、应用持续时间（24或48小时）和移植部位（尾侧静脉或肾内）。在进行治疗后，在第8天和第31天，每组中的四只动物经受左肾切除术用于组织学研究。在组织学上，在用PBS处理的组中观察到更大量的细胞碎片和缺乏上皮的小管以及更高程度的坏死，而在用ADSC处理的组中则相反，结论：ADSC移植对肾小管上皮细胞替代坏死组织，促进肾实质血管化，恢复器官功能有积极作用。
Purpose: To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cellMethods: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies.Results: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury.Conclusion: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.