Structure and spatial expression of the Manduca sexta MSCP14.6 cuticle gene
Structure and spatial expression of the Manduca sexta MSCP14.6 cuticle gene
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DOI：
10.1016/s0965-1748(96)00090-2
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发表时间：
1997-03-01
影响因子：
3.8
通讯作者：
Riddiford, LM
中科院分区：
文献类型：
--
作者：
Rebers, JE;Niu, J;Riddiford, LM
The genomic and cDNA sequences of the MSCP14.6 gene , which encodes a Manduca sexta cuticle protein, have been determined, The genomic sequence presented here includes 1428 N of 5' flanking DNA, 1587 N of transcribed DNA, and 465 N of 3' flanking DNA. The intron/exon boundaries mapped by comparison of these sequences were confirmed by primer extension and S1 nuclease mapping, Prior work showed that the RNA produced by this gene is regulated both temporally and spatially during larval, pupal, and adult development, and also showed that both 20-hydroxyecdysone (20E) and juvenile hormone (JH) are important in controlling the amount of RNA present in the epidermis (Riddiford et al., 1986), In situ hybridizations show that MSCP14.6 is expressed throughout the epidermis in larvae and is spatially restricted in pharate pupal and pharate adult stages, The results presented here delimiting the transcription unit and 5' flanking DNA of the MSCP14.6 gene will facilitate further research to determine the DNA elements necessary for regulation of gene expression. (C) 1997 Elsevier Science Ltd.
