Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential.
Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential.
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哺乳动物中成血管细胞的分离：具有生肌潜力的周细胞亚群。
DOI：
10.1007/978-1-0716-1056-5_11
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Sampaolesi,Maurilio
中科院分区：
文献类型：
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作者：
Giacomazzi,Giorgia;Giovannelli,Gaia;Rotini,Alessio;Costamagna,Domiziana;Quattrocelli,Mattia;Sampaolesi,Maurilio
成中膜血管细胞（MAB）是表达周细胞标志物的血管相关干细胞，最初从胚胎背主动脉分离。从出生后骨骼肌和心脏的小血管中，有可能分离出具有与胚胎MABs相似特征的细胞。成年MAB具有自我更新的能力，并在适当的培养条件下分化成中胚层谱系的细胞类型。到目前为止，MABs的起源以及与其他肌肉干细胞的关系仍然存在争议。最近，在I-II期临床试验中，动脉内HLA匹配的MABs被证明是相对安全的。关于MAB纯群体的新信息是可取的，并且其治疗潜力的实施对于接近基于MAB的治疗的功效是强制性的。本章概述了目前用于啮齿动物、犬、人和马成年MAB分离和表征的技术。
Mesoangioblasts (MABs) are vessel-associated stem cells that express pericyte markers and are originally isolated from the embryonic dorsal aorta. From postnatal small vessels of skeletal muscle and heart, it is possible to isolate cells with similar characteristics to embryonic MABs. Adult MABs have the capacity to self-renew and to differentiate into cell types of mesodermal lineages upon proper culture conditions. To date, the origin of MABs and the relationship with other muscle stem cells are still debated. Recently, in a phase I–II clinical trial, intra-arterial HLA-matched MABs were proved to be relatively safe. Novel information on MAB pure populations is desirable, and implementation of their therapeutic potential is mandatory to approach efficacy in MAB-based treatments. This chapter provides an overview of the current techniques for isolation and characterization of rodent, canine, human, and equine adult MABs.