Genome-wide identification and expression analysis of StTCP transcription factors of potato (Solarium tuberosum L.)
Genome-wide identification and expression analysis of StTCP transcription factors of potato (Solarium tuberosum L.)
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马铃薯(Solarium tuberosum L.)StTCP转录因子的全基因组鉴定及表达分析
DOI：
10.1016/j.compbiolchem.2018.11.009
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发表时间：
2019-02-01
影响因子：
3.1
通讯作者：
Si, Huaijun
中科院分区：
文献类型：
--
作者：
Wang, Yapeng;Zhang, Ning;Si, Huaijun
植物特异性TCP转录因子在许多生物学过程中发挥着重要作用，已在多种植物中得到鉴定和分析。然而，目前还没有马铃薯TCP家族基因的系统研究。已经进行了。本研究共鉴定了31个马铃薯非冗余TCP转录因子，并将其分为两个亚家族，包括三个不同的亚支。通过同线性和系统发育分析，鉴定了拟南芥、水稻、马铃薯和番茄中的各种直链TCP基因。蛋白质基序分析表明，StTCP在同一分支共享相似的保守基序结构。基因结构分析表明，几乎所有的StTCPs显示高度保守的外显子-内含子组织。对StTCP基因启动子区的分析表明，StTCP基因启动子区存在多个顺式作用元件，这些元件参与植物的生长发育、激素反应以及胁迫反应。StTCP基因表达谱分析结果表明，它们具有组织特异性表达模式，提示它们的分化功能。定量RT-PCR（qRT-PCR）结果表明，7个StTCP基因在马铃薯块茎休眠解除过程中表达显著上调，其中一些特异的StTCP基因对不同的非生物胁迫和多种激素有强烈的响应，表明它们在马铃薯的生长发育过程中起着重要作用。本研究结果为马铃薯StTCP家族基因的功能研究提供了重要的参考。
The plant-specific TCP transcription factors, which play critical roles in diverse aspects of biological processes, have been identified and analyzed in various plant species. However, no systematical study of TCP family genes in potato (Solanum tuberosum L.) has been undertaken. In this study, a total of 31 non-redundant TCP transcription factors of potato were identified and divided into two subfamilies including three distinct subclades. The various orthologous TCP genes in Arabidopsis, rice, potato and tomato were identified using synteny and phylogenetic analysis. Protein motif analysis demonstrated that StTCPs in the same subclade shared similar conserved motif structures. Gene structure analysis showed that almost all StTCPs displayed highly conserved exon-intron organization. The analysis of StTCP gene promoter regions revealed that multiple cis-acting elements were involved in plant growth, development, hormone responses as well as stress responses. The result of StTCP gene expression profiles showed they had tissue-specific expression patterns which implied their differentiated functions. According to the results of quantitative RT-PCR (qRT-PCR), 7 StTCP genes were dramatically upregulated during the release of tuber dormancy and some specific StTCP genes were strongly responding to different abiotic stresses and multiple hormones, which suggested they had important roles in potato growth and development processes. The results of our findings could provide comprehensive insights in StTCP family genes of potato for further functional investigations.