A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T
A new soybean maturity and photoperiod-sensitivity locus linked to E1 and T
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DOI：
10.2135/cropsci2001.413698x
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发表时间：
2001-05-01
期刊：
影响因子：
2.3
通讯作者：
Voldeng, HD
中科院分区：
文献类型：
--
作者：
Cober, ER;Voldeng, HD
在短季大豆[Glycine mar（L.）梅尔。]。本研究的目的是确定是否有一个单一的基因座控制早熟，如果有一个新的基因座连接到E1和T，或者，在E1基因座的第三个等位基因。在“Harosoy”等株系0 T 89 -5（e3 e3 e4 e4）和0 T 94 -47之间进行杂交。PI 196529是开发OT 94 -47的回交程序中的早熟供体。共229个F-2植物和父母下生长的20小时光周期产生的白炽灯。OT 94 -47在43 +/- 1.4 d开花，OT 89 -5在57 +/- 4.2 d开花较晚，F2群体符合3晚：1早花比例。早花F2植物产生的F-3家族的花类似于OT 94 -47。晚花F2植物的开花日期分离或开花类似于OT 89 -5。为了测试与E1的等位性和与T的连锁，在OT 93 -26（E1 E1 e3 e3 e4 e4 TT）和OT 94 -47之间进行杂交。F_2植株被分类为亲本类型或中间类型，并且在成熟度上与OT 89 -5相当。在376个F3后代行中记录了成熟度和绒毛颜色。数据不符合单位点模型或两位点显性上位性模型（12：3：1）。E7等位基因对e7等位基因部分但不完全显性。因此，提出了一个新的位点E7。使用F3分离数据，T和E1之间的连锁估计为1.3 +/-0.6厘摩（cM）。E1和E7之间的连锁估计为6.2 cM，E7和T之间的连锁估计为3.9 cM。E7是一个与E1和T紧密连锁的新的开花、成熟和光周期敏感位点。与e7 e7相比，E7 E7导致较晚的开花和成熟，以及对白炽灯产生的长光周期的敏感性。
An association between early maturity and tawny pubescence has been observed in short-season soybean [Glycine mar (L.) Merr.]. The objectives of this study were to determine if a single locus controls early maturity; and if there is a new locus linked to E1 and T or, alternatively, a third allele at the E1 locus. A cross was made between 'Harosoy' isolines OT89-5 (e3e3 e4e4) and OT94-47. PI 196529 was the donor of early maturity in the backcrossing program which developed OT94-47. A total of 229 F-2 plants and the parents were grown under 20-h photoperiods produced by incandescent lamps. OT94-47 flowered in 43 +/- 1.4 d, OT89-5 flowered later in 57 +/- 4.2 d, and the F2 population fit a 3 late: 1 early flowering ratio. Early-flowering F2 plants produced F-3 families that flowered similarly to OT94-47. Later-flowering F2 plants either segregated for flowering date or flowered similarly to OT89-5. To test for allelism with E1 and linkage with T, a cross was made between OT93-26 (E1E1 e3e3 e4e4 TT) and OT94-47. F2 plants were classified as parental types or intermediate and equivalent to OT89-5 in maturity. Maturity and pubescence color were recorded in 376 F3 progeny rows. The data did not fit a single locus model or a two loci dominant epistasis model (12:3:1). The E7 allele was partially, but not completely, dominant over the e7 allele. Therefore, a new locus E7 is proposed. Using the F3 segregation data, linkage between T and E1 was estimated to be 1.3 +/-0.6 centimorgan (cM). Linkage between E1 and E7 was estimated to be 6.2 cM and linkage between E7 and T was estimated to be 3.9 cM. E7 is a new flowering, maturity, and photoperiod sensitivity locus tightly linked to both E1 and T. E7E7 results in later flowering and maturity, and sensitivity to long photoperiods produced by incandescent lamps when compared to e7e7.