Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds
Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds
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DOI：
10.1073/pnas.97.12.6728
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发表时间：
2000-06-06
影响因子：
11.1
通讯作者：
Zhang, SG
中科院分区：
文献类型：
--
作者：
Holmes, TC;de Lacalle, S;Zhang, SG
描述了一种新型的自组装肽（sapeptide）支架，其作为神经突生长和突触形成的底物。这些基于肽的支架可通过使用化学或生物技术合成进行分子设计。它们可以根据各种应用进行定制。sapeptide支架是通过在生理条件下离子自互补β-折叠寡肽的自发组装形成的，产生水凝胶材料。该支架可以支持神经元细胞的附着和分化以及广泛的神经突生长。此外，它们是附着的神经元之间功能性突触形成的容许底物。原代大鼠神经元在这种支架表面原位形成活跃的突触，表明这些支架可用于组织工程应用。在培养中具有附着细胞的漂浮的sapeptide支架可以容易地从一个环境运输到另一个环境。此外，这些肽在引入动物体内时不会引起可测量的免疫应答或组织炎症。通过分子设计和自组装产生的这些生物材料可以被开发为用于组织修复和组织工程的生物相容性支架。
A new type of self-assembling peptide (sapeptide) scaffolds that serve as substrates for neurite outgrowth and synapse formation is described. These peptide-based scaffolds are amenable to molecular design by using chemical or biotechnological syntheses. They can be tailored to a variety of applications. The sapeptide scaffolds are formed through the spontaneous assembly of ionic self-complementary beta-sheet oligopeptides under physiological conditions, producing a hydrogel material. The scaffolds can support neuronal cell attachment and differentiation as well as extensive neurite outgrowth. Furthermore, they are permissive substrates for functional synapse formation between the attached neurons. That primary rat neurons form active synapses on such scaffold surfaces in situ suggests these scaffolds could be useful for tissue engineering applications. The buoyant sapeptide scaffolds with attached cells in culture can be transported readily from one environment to another. Furthermore, these peptides did not elicit a measurable immune response or tissue inflammation when introduced into animals. These biological materials created through molecular design and self assembly may be developed as a biologically compatible scaffold for tissue repair and tissue engineering.