MORPHOGENESIS IN PINOID MUTANTS OF ARABIDOPSIS-THALIANA
MORPHOGENESIS IN PINOID MUTANTS OF ARABIDOPSIS-THALIANA
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DOI：
10.1046/j.1365-313x.1995.8040505.x
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发表时间：
1995-10-01
期刊：
影响因子：
7.2
通讯作者：
SMYTH, DR
中科院分区：
文献类型：
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作者：
BENNETT, SRM;ALVAREZ, J;SMYTH, DR
选择了一系列拟南芥突变体，其中花序茎伸长，但失去了在其侧翼产生花原基的能力。突变体分为两类，进一步发生的针形成的突变体和突变在一个新的位点命名为pinoid。除了引起花序缺陷外，松状突变还导致花器官、子叶和叶发育的多效性缺陷。大多数变化涉及产生的器官的数量，而不是它们的分化，这表明Pinnut控制着分生组织发育的早期一般步骤。对于花序和花，pinoid突变体缺陷类似于在pin-formed突变体中所见的缺陷，但对于子叶和叶，pinoid突变体缺陷不同，这表明这两个基因具有独立但重叠的功能。在极性生长素运输的缺陷牵连在针形成的突变体表型，但在年轻的花序茎，即使是最强的松状突变体，它发生在接近野生型的水平。只有在茎停止伸长后才明显减少。因此，这是可能的极性生长素运输是次要的影响，而不是由pinidine基因产物直接控制的松类突变体。即便如此，双突变体研究表明，由PINESTINE控制的过程与由生长素抗性1基因指定的过程重叠，这表明PINESTINE在生长素相关过程中起一定作用。
A series of mutants of Arabidopsis thaliana was selected in which the inflorescence stem elongates but loses the ability to produce flower primordia on its flanks. Mutants fell into two classes, further occurrences of pin-formed mutants and mutations at a new locus named pinoid. As well as causing inflorescence defects, pinoid mutations result in pleiotropic defects in the development of floral organs, cotyledons and leaves. Most changes involve the number of organs produced rather than their differentiation suggesting that PINOID controls an early general step in meristem development. pinoid mutant defects are similar to those seen in pin-formed mutants for inflorescences and flowers, but different for cotyledons and leaves indicating that the two genes have separate but overlapping functions. A defect in polar auxin transport is implicated in the pin-formed mutant phenotype, but in young inflorescence stems of even the strongest pinoid mutants it occurs at close to wild-type levels. It is markedly reduced only after stems have ceased elongating. Thus, it is likely that polar auxin transport is secondarily affected in pinoid mutants rather than being directly controlled by the PINOID gene product. Even so, double mutant studies indicate that the process controlled by PINOID overlaps with that specified by the AUXIN RESISTANT1 gene, suggesting that PINOID plays some role in an auxin-related process.