ISOLATION OF MOUSE-LIVER CELLS - PERFUSION TECHNIQUE AND METABOLIC EVALUATION
ISOLATION OF MOUSE-LIVER CELLS - PERFUSION TECHNIQUE AND METABOLIC EVALUATION
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DOI：
10.1159/000128339
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发表时间：
1983-01-01
影响因子：
1.6
通讯作者：
LUNDHOLM, K
中科院分区：
文献类型：
--
作者：
EDSTROM, S;EKMAN, L;LUNDHOLM, K
建立了一种胶原酶灌注分离完整小鼠肝细胞的方法。小鼠肝脏经心脏原位灌注。细胞代谢评价为呼吸活动和蛋白质合成。结果与报道的大鼠肝细胞分离的结果一致。缺点是每只动物获得的肝细胞产量低。这里描述的程序似乎提供了一种方便的方法，通过这种方法可以获得适合于急性代谢实验的分离小鼠肝细胞。
A procedure was developed for isolation of intact mouse liver cells by collagenase perfusion. Mouse livers were perfused in situ via the heart. The cell metabolism was evaluated as respiratory activity and protein synthesis. The results agree with those which have been reported for isolated rat liver cells. A shortcoming was the low yield of liver cells obtained per animal. The procedure described here seems to offer a convenient means by which it is possible to obtain isolated mouse liver cells which are appropriate for acute metabolic experiments.