Raman Reporter-Coupled Ag(core)@Au(shell) Nanostars for in Vivo Improved Surface Enhanced Raman Scattering Imaging and Near-infrared-Triggered Photothermal Therapy in Breast Cancers.
Raman Reporter-Coupled Ag(core)@Au(shell) Nanostars for in Vivo Improved Surface Enhanced Raman Scattering Imaging and Near-infrared-Triggered Photothermal Therapy in Breast Cancers.
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DOI：
10.1021/acsami.5b04548
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发表时间：
2015-07
影响因子：
9.5
通讯作者：
L. Zeng;Yuanwei Pan;Shouju Wang;Xin Wang;Xinmei Zhao;Wenzhi Ren;Guangming Lu;A. Wu
中科院分区：
文献类型：
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作者：
L. Zeng;Yuanwei Pan;Shouju Wang;Xin Wang;Xinmei Zhao;Wenzhi Ren;Guangming Lu;A. Wu
贵金属纳米材料作为表面增强拉曼散射（Sers）成像和光热治疗（PTT）的治疗诊断系统被广泛研究。然而，开发高性能、高稳定性和低毒性的多功能纳米探针仍然是一个重大挑战。在这项工作中，合成了拉曼报告基因（DTTC）偶联的Agcore@Aushell纳米星（Ag@Au-DTTC），并研究了其在体内改善的Sers成像和近红外（NIR）触发的乳腺癌PTT。DTTC的两步偶联明显提高了Sers信号，同时在Ag纳米颗粒表面包覆Au纳米星也降低了纳米颗粒的细胞毒性。所制备的Ag@Au-DTTC纳米星具有较高的光稳定性和良好的光热性能，在808 nm激光照射下，光热转换效率高达79.01%。Ag@Au-DTTC纳米星的体Sers测试结果表明，与纯DTTC相比，在MCF-7细胞和MCF-7荷瘤裸鼠体内均能观察到位于508、782、844、1135、1242、1331、1464、1510和1580 cm（-1）的多个尖锐和狭窄的拉曼峰。在14天的治疗中，用Ag@Au-DTTC纳米星注射并用808 nm激光照射的MCF-7荷瘤裸鼠的肿瘤体积几乎消失。该研究表明，所制备的Ag@Au-DTTC纳米星可以是用于改善低风险乳腺癌的Sers成像和NIR触发的PTT的优异的多功能试剂。
Noble-metal nanomaterials were widely investigated as theranostic systems for surface enhanced Raman scattering (SERS) imaging, and also for photothermal therapy (PTT) of cancers. However, it was still a major challenge to explore multifunctional nanoprobes with high performance, high stability, and low toxicity. In this work, Raman reporter (DTTC)-coupled Agcore@Aushell nanostars (Ag@Au-DTTC) were synthesized and investigated for in vivo improved SERS imaging and near-infrared (NIR)-triggered PTT of breast cancers. By the two-step coupling of DTTC, the SERS signal was improved obviously, and the cytotoxicity of nanoparticles was also decreased by coating Au nanostars onto Ag nanoparticles. The as-prepared Ag@Au-DTTC nanostars showed high photostability and excellent photothermal performance, in which the photothermal conversion efficiency was up to 79.01% under the irradiation of an 808 nm laser. The in vitro and in vivo SERS measurements of Ag@Au-DTTC nanostars showed that the many sharp and narrow Raman peaks located at 508, 782, 844, 1135, 1242, 1331, 1464, 1510, and 1580 cm(-1) could be obviously observed in MCF-7 cells and in MCF-7 tumor-bearing nude mice, compared with that in pure DTTC. In 14-day treatments, the tumor volume of MCF-7 tumor-bearing nude mice injected with Ag@Au-DTTC nanostars and irradiated by an 808 nm laser almost disappeared. This study demonstrated that the as-prepared Ag@Au-DTTC nanostars could be excellent multifunctional agents for improved SERS imaging and NIR-triggered PTT of breast cancers with low risk.