Measuring hemoglobin spectra: searching for carbamino-hemoglobin.
Measuring hemoglobin spectra: searching for carbamino-hemoglobin.
复制标题
测量血红蛋白光谱：寻找卡巴米诺 - 丝状球蛋白。
DOI：
10.1117/1.jbo.25.10.105001
复制
发表时间：
2020-10
影响因子：
3.5
通讯作者：
Théron M
中科院分区：
文献类型：
--
作者：
Dervieux E;Bodinier Q;Uhring W;Théron M
意义：动脉二氧化碳分压是一个临床相关变量。然而，它的测量需要动脉采血或笨重而昂贵的经皮测量仪。虽然用分光光度法测定血红蛋白种类--如氧合血红蛋白(HHb)和脱氧血红蛋白(HHb)--为脉搏血氧仪的发展提供了可能，但对血液含量的测量还没有涉及到最小的不适感。目的：表征文献中缺失的人碳氨基-血红蛋白吸收光谱。为经皮非侵入性测量提供理论基础。方法：使用一种基于眼压测量的方法来获取气体平衡、溶解、稀释的人血。平衡是在氮素()和环境空气中进行的。在235至1000 nm范围内进行了分光光度测量。脉搏血氧仪的理论背景也得到了发展。结果：HHb和HHb的吸收光谱非常接近，与最先进的HHb的吸收光谱相当。上述理论背景导致对受试者血液中的测量量的估计相对误差在30%以上。辅助测量表明，乙二胺四乙酸的使用不会干扰分光光度测量，而焦亚硫酸钠则会干扰。结论：首次测定了其吸收光谱。这样的频谱接近于HHB的频谱，使用基于脉搏血氧仪理论的理论背景进行非侵入性测量似乎极具挑战性。
Significance: The arterial carbon dioxide () partial pressure is a clinically relevant variable. However, its measurement requires arterial blood sampling or bulky and expensive transcutaneous meters. While the spectrophotometric determination of hemoglobin species—such as oxy-hemoglobin () and deoxy-hemoglobin (HHb)—allowed for the development of pulse oximetry, the measurement of blood content with minimal discomfort has not been addressed yet. Aim: Characterizing human carbamino-hemoglobin () absorption spectrum, which is missing from the literature. Providing the theoretical background that will allow for transcutaneous, noninvasive measurements. Approach: A tonometry-based approach was used to obtain gas-equilibrated, lysed, diluted human blood. Equilibration was performed with both , dinitrogen (), and ambient air. Spectrophotometric measurements were carried out on the 235- to 1000-nm range. A theoretical background was also derived from that of pulse oximetry. Results: The absorption spectra of both and HHb were extremely close and comparable with that of state-of-the-art HHb. The above-mentioned theoretical background led to an estimated relative error above 30% on the measured amount of in a subject’s blood. Auxiliary measurements revealed that the use of ethylene diamine tetraacetic acid did not interfere with spectrophotometric measurements, whereas sodium metabisulfite did. Conclusions: absorption spectrum was measured for the first time. Such spectrum being close to that of HHb, the use of a theoretical background based on pulse oximetry theory for noninvasive measurement seems extremely challenging.