Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system.
Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system.
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DOI：
10.1016/j.envpol.2016.09.074
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发表时间：
2017
影响因子：
8.9
通讯作者：
P. Maršík;M. Sisa;O. Lacina;K. Moťková;L. Langhansová;J. Rezek;Tomáš Vaněk
中科院分区：
文献类型：
--
作者：
P. Maršík;M. Sisa;O. Lacina;K. Moťková;L. Langhansová;J. Rezek;Tomáš Vaněk
采用悬浮培养的拟南芥研究了植物对布洛芬（IBU）的吸收和代谢。几乎所有加入培养基（200 μM）的IBU在5天内代谢或结合至不溶性结构。通过液相色谱-高分辨率质谱（LC-HRMS）分析确定了300多种代谢物，其中大部分是首次报道的植物。虽然羟基化的衍生物形成的异丁基侧链上的氧化是IBU降解的主要第一步产品，这些产品与糖，甲基和氨基酸基团的共轭物是占主导地位的代谢产物的文化。总添加IBU的主要部分（81%）累积在可提取的细胞内池中，而培养基部分仅含19%。不溶性细胞壁结合IBU的量可忽略不计（总IBU的0.005%）。
The uptake and metabolism of ibuprofen (IBU) by plants at the cellular level was investigated using a suspension culture ofA. thaliana. Almost all IBU added to the medium (200 μM) was metabolized or bound to insoluble structures in 5 days. More than 300 metabolites were determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis, and most of these are first reported for plants here. Although hydroxylated derivatives formed by oxidation on the isobutyl side chain were the main first-step products of IBU degradation, conjugates of these products with sugar, methyl and amino acid groups were the dominant metabolites in the culture. The main portion of total added IBU (81%) was accumulated in the extractable intracellular pool, whereas the cultivation medium fraction contained only 19%. The amount of the insoluble cell-wall-bound IBU was negligible (0.005% of total IBU).