Sub-micron texturing for reducing platelet adhesion to polyurethane biomaterials
Sub-micron texturing for reducing platelet adhesion to polyurethane biomaterials
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DOI：
10.1002/jbm.a.30554
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发表时间：
2006-03-01
影响因子：
4.9
通讯作者：
Siedlecki, CA
中科院分区：
文献类型：
--
作者：
Milner, KR;Snyder, AJ;Siedlecki, CA
血小板粘附是血液接触医疗器械上血栓形成的关键事件。已经证明，材料表面化学性质的改变可以减少血小板粘附。在这项工作中，假设亚微米表面纹理也可能通过减少血小板可以接触的材料表面积来减少粘附，从而降低与粘附配体相互作用的可能性。使用不改变材料表面化学性质的复制成型技术，将聚醚（聚氨酯脲）制成两种不同尺寸的亚微米柱。使用转盘系统在 0-67 dyn/cm(2) 的生理相关剪切应力范围内评估血小板的粘附力。对血小板进行免疫荧光标记，并在光滑和有纹理的样品上比较粘附力。血小板粘附在 0 至 5 dyn/cm(2) 的低剪切应力下达到最大，并且观察到亚微米纹理可减少该范围内的血小板粘附。此外，非粘附血小板在暴露于纹理样品后并未表现出大规模激活。我们得出的结论是，具有亚血小板尺寸的表面纹理可以减少低剪切应力下血小板从血浆到聚醚（聚氨酯脲）的粘附。 (C) 2005 年 Wiley 期刊公司。
Platelet adhesion is a key event in thrombus development on blood-contacting medical devices. It has been demonstrated that changes to the chemistry of a material surface can reduce platelet adhesion. In this work, it is hypothesized that sub-micron surface textures may also reduce adhesion via a decrease in the surface area of material with which platelets can make contact, and hence a decreased probability of interaction with adhesive ligands. A polyether(urethane urea) was textured with two different sizes of sub-micron pillars using a replication molding technique that did not alter the material surface chemistry. Adhesion of platelets was assessed in a physiologically relevant shear stress range of 0-67 dyn/cm(2) using a rotating disk system. Platelets were immunofluorescently labeled and adhesion was compared on smooth and textured samples. Platelet adhesion was greatest at low shear stress ranging from 0 to 5 dyn/cm(2), and sub-micron textures were observed to reduce platelet adhesion in this range. Additionally, non-adherent platelets did not demonstrate large-scale activation after exposure to textured samples. We conclude that surface textures with sub-platelet dimensions may reduce platelet adhesion from plasma to polyether(urethane urea) at low shear stress. (C) 2005 Wiley Periodicals, Inc.