ALTERED KERATIN EXPRESSION IN BUCCAL MUCOSAL SQUAMOUS-CELL CARCINOMA
ALTERED KERATIN EXPRESSION IN BUCCAL MUCOSAL SQUAMOUS-CELL CARCINOMA
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DOI：
10.1111/j.1600-0714.1989.tb00398.x
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发表时间：
1989-05-01
影响因子：
3.3
通讯作者：
BHISEY, AN
中科院分区：
文献类型：
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作者：
VAIDYA, MM;BORGES, AM;BHISEY, AN
应用SDS-PAGE、免疫印迹和双向电泳技术，分析了14例中分化和12例高分化颊粘膜鳞癌的细胞角蛋白谱。尽可能将这些与正常颊粘膜和周围区域的模式进行比较。正常颊粘膜表达角蛋白4（59 Kd）、5（58 Kd）、13（54 Kd）和14（50 Kd）。角蛋白4号（59 Kd）和14号（50 Kd）在26例肿瘤中有20例表达，而角蛋白5号（58 Kd）和13号（54 Kd）在大多数肿瘤中不表达。角蛋白1号（67 Kd）和16号（48 Kd）在9例高分化肿瘤中表达异常。角蛋白17（46 kd）和18（45 kd）在14例中分化肿瘤中分别有10例和8例表达。6个肿瘤表现为肺泡粘膜受累，表达了一些角蛋白表达的正常对应。它们改变的表达与前面所述的分化模式一致。角蛋白5和13的不表达似乎是恶性转化的结果，并且在大多数肿瘤中可见，而异常角蛋白的出现似乎与肿瘤的分化程度更相关。
Cytokeratin pattern was analyzed in 14 moderately differentiated and 12 well-differentiated squamous cell carcinomas of buccal mucosa by SDS-PAGE, immunoblotting and two dimensional electrophoresis. These were compared with patterns of normal buccal mucosa and surrounding areas whenever possible. Normal buccal mucosa expresses keratin No. 4 (59Kd), 5 (58Kd), 13 (54Kd) and 14 (50Kd). Keratin No. 4 (59Kd) and 14 (50Kd) were expressed by 20 of 26 tumors studied, while many of the tumors did not express keratins No. 5 (58Kd) and 13 (54Kd). Keratin No. 1 (67Kd) and 16 (48Kd) were aberrantly expressed by 9 well-differentiated tumors. Keratin No. 17 (46kd) and 18 (45Kd) were expressed by 10 and 8 tumors of 14 moderately differentiated tumors. Six tumors which showed involvement of alveolar mucosa, expressed some keratins expressed by its normal counterpart. Their altered expression was consistent with the differentiation pattern as stated earlier. Non-expression of keratins 5 and 13 seems to be the result of malignant transformation and is seen in the majority of tumors, while appearance of aberrant keratins seems to be related more to the degree of differentiation of the tumor.