Human mammary cell survival following ionizing radiation.
Human mammary cell survival following ionizing radiation.
复制标题
电离辐射后人类乳腺细胞的存活。
DOI：
10.1080/09553008314550821
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Gould,MN
中科院分区：
文献类型：
--
作者：
Cathers,LE;Gould,MN
我们已经开发并评估了培养正常人乳腺细胞的确定基质和上皮细胞群的方法。这些细胞群用于生成辐射剂量/存活曲线。上皮细胞群体需要特定的激素、生长因子和条件培养基，以及成纤维细胞饲养层用于克隆生长。基质细胞在不太复杂的培养基中生长良好。正常人乳腺的间质和实质细胞群的特征在于光镜和电子显微镜，免疫组织化学人纤连蛋白染色，γ-谷氨酰转肽酶组织化学染色，和细胞大小。使用来自四个供体的细胞生成存活曲线。上皮细胞的平均D 0为122 cGy，平均值为2.4。基质细胞的平均D 0和n值分别为114 cGy和2.0。将人乳腺上皮细胞的存活率与大鼠乳腺细胞的存活率进行比较。两个物种的D 0值基本相同，而人类上皮细胞的D 0值较低。这种thn值的差异可能是物种对辐射的特异性反应，或者可能仅仅反映了用于生成存活曲线的两种测定系统的差异。
We have developed and evaluated methods of culturing defined stromal and epithelial populations of normal human breast cells. These cell populations were used to generate radiation dose/survival curves. The epithelial cell population required specific hormones, growth factors, and conditioned media, as well as fibroblast feeder layers for clonal growth. Stromal cells grew well in a less complex medium. The stromal and parenchymal cell populations of the normal human breast were characterized by light and electron microscopy, immunohistochemical human fibronectin staining, gamma glutamyltranspeptidase histochemical staining, and cell sizing. Survival curves were generated using cells from four donors. The averageD0for epithelial cells was 122 cGy, with an averagenvalue of 2·4. The averageD0andnvalues for stromal cells were 114 cGy and 2·0. The survival of human breast epithelial cells is compared to that of the cells of the rat mammary gland. TheD0values of both species are essentially the same, while thenvalue for human epithelial cells is lower. This difference in thenvalue may be a species specific response to radiation, or may merely reflect a difference in the two assay systems used to generate the survival curves.