Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells
Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells
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DOI：
10.1073/pnas.94.8.3789
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发表时间：
1997-04-15
影响因子：
11.1
通讯作者：
Soriano, P
中科院分区：
文献类型：
--
作者：
Zambrowicz, BP;Imamoto, A;Soriano, P
ROSA beta geoZ6 (ROSA26) 小鼠品系是通过胚胎干细胞中随机逆转录病毒基因捕获产生的。 ROSA26 组织的染色和造血细胞的荧光激活细胞分选仪-Gal 分析证明了前病毒 β geo 报告基因的普遍表达，骨髓移植实验说明了该菌株在嵌合体和移植研究中的一般用途。基因捕获载体已整合到产生三个转录本的区域中。 ROSA26 纯合动物中丢失的两个转录本源自共同的启动子并具有相同的 5' 末端，但都不包含显着的 ORF。第三个转录物源自反向链，与非编码转录物之一共享反义序列。第三个转录物可能编码一种至少有 505 个氨基酸的新蛋白质，该蛋白质在人类和秀丽隐杆线虫中是保守的。
The ROSA beta geoZ6 (ROSA26) mouse strain was produced by random retroviral gene trapping in embryonic stem cells. Staining of ROSA26 tissues and fluorescence-activated cell sorter-Gal analysis of hematopoietic cells demonstrates ubiquitous expression of the proviral beta geo reporter gene, and bone marrow transfer experiments illustrate the general utility of this strain for chimera and transplantation studies. The gene trap vector has integrated into a region that produces three transcripts. Two transcripts, lost in ROSA26 homozygous animals, originate from a common promoter and share identical 5' ends, but neither contains a significant ORF. The third transcript, originating from the reverse strand, shares antisense sequences with one of the noncoding transcripts. This third transcript potentially encodes a novel protein of at least 505 amino acids that is conserved in humans and in Caenorhabditis elegans.