Role of cyclooxygenase-1 and-2 in endothelium-dependent contraction of atherosclerotic mouse abdominal aortas
Role of cyclooxygenase-1 and-2 in endothelium-dependent contraction of atherosclerotic mouse abdominal aortas
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环氧合酶-1和-2在动脉粥样硬化小鼠腹主动脉内皮依赖性收缩中的作用
DOI：
10.1111/1440-1681.12501
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发表时间：
2016-01-01
影响因子：
2.9
通讯作者：
Zhou, Yingbi
中科院分区：
文献类型：
--
作者：
Li, Shasha;Liu, Bin;Zhou, Yingbi
本研究的目的是确定环氧合酶（考克斯）-1或-2在动脉粥样硬化条件下内皮依赖性收缩中的作用。采用高脂高胆固醇饲料喂养apoE基因敲除（apoE（-/-））小鼠和考克斯-1（-/-）（apoE（-/-）-考克斯-1（-/-））小鼠，诱导动脉粥样硬化。分离主动脉（腹部或整个切片）进行功能和/或生化分析。在非动脉粥样硬化的条件下，毒蕈碱受体激动剂乙酰胆碱（ACh）诱发内皮依赖性，COX介导的收缩后，NO合酶（NOS）抑制腹主动脉环动脉粥样硬化apoE（-/-）小鼠。有趣的是，考克斯-1抑制不仅消除了正常环的收缩，而且减少了斑块环的收缩。因此，只有轻微收缩（
The objective of this study was to determine the role of cyclooxygenase (COX)-1 or -2 in endothelium-dependent contraction under atherosclerotic conditions. Atherosclerosis was induced in apoE knockout (apoE(-/-)) mice and those with COX-1(-/-) (apoE(-/-)-COX-1(-/-)) by feeding with high fat and cholesterol food. Aortas (abdominal or the whole section) were isolated for functional and/or biochemical analyses. As in non-atherosclerotic conditions, the muscarinic receptor agonist acetylcholine (ACh) evoked an endothelium-dependent, COX-mediated contraction following NO synthase (NOS) inhibition in abdominal aortic rings from atherosclerotic apoE(-/-) mice. Interestingly, COX-1 inhibition not only abolished such a contraction in rings showing normal appearance, but also diminished that in rings with plaques. Accordingly, only a minor contraction (