Dual function of (-)-epigallocatechin gallate (EGCG) in healthy human lymphocytes
Dual function of (-)-epigallocatechin gallate (EGCG) in healthy human lymphocytes
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DOI：
10.1016/j.canlet.2005.10.021
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发表时间：
2006-09-28
期刊：
影响因子：
9.7
通讯作者：
Morimoto, Kanehisa
中科院分区：
文献类型：
--
作者：
Kanadzu, Masaki;Lu, Yuquan;Morimoto, Kanehisa
（-）-表没食子儿茶素没食子酸酯（EGCG）是一种儿茶素多酚成分，是绿色茶提取物的主要成分。近年来，其抗氧化作用受到越来越多的关注。然而，一些研究报道了EGCG的氧化作用，表明EGCG具有抗氧化和促氧化的双重功能。在这项研究中，我们研究了EGCG对健康人全血淋巴细胞和纯化的血液淋巴细胞的影响，使用单细胞凝胶电泳（SCG）分析。结果表明，EGCG在10（-8）~ 10（-5）M浓度范围内抑制全血淋巴细胞DNA链断裂，而在10（-3）M浓度范围内诱导DNA链断裂。此外，浓度为10（-6）-10（-4）M的EGCG抑制由博莱霉素（BLM）和H_2O_2诱导的全血淋巴细胞DNA链断裂。在10（-6）~ 10（-4）M浓度范围内，EGCG可增加淋巴细胞DNA链的断裂，而在10（-8）~ 10（-7）M浓度范围内，EGCG可抑制BLM诱导的淋巴细胞DNA链断裂。由此推测，EGCG对BLM和H_2O_2诱导的人淋巴细胞DNA链断裂具有抑制作用，可能与其具有抗氧化和促氧化双重作用有关。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
(-)-Epigallocatechin gallate (EGCG), a catechin polyphenol component, is the main ingredient of green tea extract. Recently, increasing attention has been given to its anti-oxidant effects. However, several studies reported the oxidative effects of EGCG, suggesting that EGCG had a dual function of anti-oxidant and pro-oxidant potentials. In this study, we examined the influences of EGCG on healthy human whole blood lymphocytes and purified blood lymphocytes using a single-cell gel electrophoresis (SCG) assay. The results showed that EGCG suppressed the DNA strand breakage in whole blood lymphocytes at concentrations from 10(-8) to 10(-5) M, while it induced DNA strand breakage at concentration of 10(-3) M. Furthermore, EGCG at concentrations of 10(-6)-10(-4) M suppressed the DNA strand breakage induced by bleomycin (BLM) and H2O2, in whole blood lymphocytes. In the same range of 10(-6)-10(-4) M, EGCG increased DNA strand breakage in purified blood lymphocytes, but suppressed the DNA breakage induced by BLM at lower concentrations from 10(-8) to 10(-7) M. From these findings, we propose that EGCG might have a dual function of anti-oxidant and pro-oxidant in healthy human lymphocytes, which would involved in its inhibitory effects against DNA strand breakage induced by BLM and H2O2. (c) 2006 Elsevier Ireland Ltd. All rights reserved.