NICKEL-CONTAINING FACTOR-F430 - CHROMOPHORE OF THE METHYLREDUCTASE OF METHANOBACTERIUM

NICKEL-CONTAINING FACTOR-F430 - CHROMOPHORE OF THE METHYLREDUCTASE OF METHANOBACTERIUM
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DOI：
10.1073/pnas.79.12.3707
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发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
通讯作者：
WOLFE, RS
WOLFE, RS
中科院分区：
其他
文献类型：
--
作者：
ELLEFSON, WL;WHITMAN, WB;WOLFE, RS

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热自养结核分枝杆菌甲基辅酶M甲基还原酶的黄色发色团为含镍因子F430。用80%的甲醇水溶液处理63ni标记的甲基还原酶，释放放射性标记物和黄色发色团；这两种性质都与一种与F430相同的化合物有关，化学计量为1 mol Ni/mol F430和2 mol F430/mol甲基化还原酶。
The yellow chromophore of the methyl-coenzyme M methylreductase of M. thermoautotrophicum was found to be the Ni-containing factor F430. Treatment of 63Ni-labeled methylreductase with 80% aqueous methanol released the radiolabel and the yellow chromophore; both properties were associated with a single compound that was identical to F430, the stoichiometry being I mol Ni/mol F430 and 2 mol F430/mol of methylreductase.