The Microbiome of the Prostate Tumor Microenvironment
The Microbiome of the Prostate Tumor Microenvironment
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DOI：
10.1016/j.eururo.2017.03.029
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发表时间：
2017-10-01
期刊：
影响因子：
23.4
通讯作者：
Salonia, Andrea
中科院分区：
文献类型：
--
作者：
Cavarretta, Ilaria;Ferrarese, Roberto;Salonia, Andrea
背景资料：基于分子的复杂微生物种群的鉴定和表征方法的出现导致了微生物发现的新时代。病理和健康前列腺组织的微生物生态系统的详细和全面的分析尚未报道。目的：表征可能与病理前列腺微环境相关的微生物组。设计、设置和参与者：在16个根治性前列腺切除术标本上评估肿瘤、肿瘤周围和非肿瘤组织的微生物组概况。结果测量和统计分析：通过大规模超深焦磷酸测序评估微生物组分析。细菌负荷表示为细菌总数的百分比。结果与局限性：3门、6纲、9目、14科、11属的植物类群间差异均超过了设定的1%阈值。肿瘤/肿瘤周围和非肿瘤前列腺标本中的特定微生物种群在某些分类水平上观察到显着差异。在属中，丙酸杆菌属（Propionibacterium spp.）是最丰富的。葡萄球菌属在肿瘤/肿瘤周围组织中更有代表性（p < 0.05）。有限数量的标本代表了一个潜在的limitation.Conclusions：前列腺中含有过多的细菌，它设置自己的腺体内的分布依赖于组织的性质，从而表明一个可能的病理生理相关性之间的组合物的局部微生物生态位和肿瘤本身的存在。未来的研究将有助于阐明这些特定细菌的作用及其作为新的生物标志物的潜力。患者摘要：病理性前列腺由特定的微生物群组成，其分布根据组织的性质而变化。这一发现为识别新的治疗方法和生物标志物开辟了有趣的前景。(C)2017年欧洲泌尿外科协会。Elsevier B. V.出版，保留所有权利。
Background: The advent of molecular-based methods of identification and characterization of complex microbial populations has led to a new era of microbial discovery. A detailed and comprehensive analysis of the microbial ecosystem of the pathologic and healthy prostate tissues has not been yet reported.Objectives: To characterize the microbiome possibly associated to the pathologic prostate microenvironment.Design, setting, and participants: The microbiome profile of tumor, peri-tumor, and nontumor tissues was assessed on 16 radical prostatectomy-specimens.Outcome measurements and statistical analysis: Microbiome analysis was assessed by massive ultradeep pyrosequencing. Bacteria load was expressed as a percentage of the total number of bacteria. The statistical significance of differences among specimen-groups was tested with Friedman's test (Dunn posthoc test) and Wilcoxon rank-sum test.Results and limitations: Three phyla, six classes, nine orders, 14 families, and 11 genera were above the set threshold value of 1%, respectively. Significant differences in specific microbial populations among tumor/peri-tumor and nontumor prostate specimens were observed at certain taxonomic levels. Among genera, Propionibacterium spp. were the most abundant. Staphylococcus spp. were more represented in the tumor/peri-tumor tissues (p < 0.05). The restricted number of specimens represents a potential limitation.Conclusions: The prostate contains a plethora of bacteria, which set themselves within the gland with a distribution dependent on the nature of the tissue, thus suggesting a possible pathophysiological correlation between the composition of the local microbial niche and the presence of the tumor itself. Future studies will help to clarify the role of these specific bacteria and their potential to be exploited as new biomarkers.Patient summary: The pathological prostate is populated by specific microbial populations, whose distribution varies according to the nature of the tissue. This finding opens interesting perspectives for the identification of novel therapeutic approaches and biomarkers. (C) 2017 European Association of Urology. Published by Elsevier B.V. All rights reserved.