A cell surface protein with herpesvirus entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus
A cell surface protein with herpesvirus entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus
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DOI：
10.1006/viro.1998.9218
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发表时间：
1998-06-20
期刊：
影响因子：
3.7
通讯作者：
Spear, PG
中科院分区：
文献类型：
--
作者：
Warner, MS;Geraghty, RJ;Spear, PG
单纯疱疹病毒1型（HSV-1）的某些突变株不能感染进入依赖于HVEM的细胞，HVEM是先前描述的疱疹病毒进入介体，在此称为疱疹病毒进入蛋白A（HveA）。这些突变病毒可以感染其他细胞，其中进入明显依赖于其他共受体。用HSV-1（科斯）Rid 1突变病毒筛选人cDNA表达文库，以确定转染质粒将抗性中国仓鼠卵巢细胞转化为对病毒进入的易感性的能力。基于该活性分离表达先前描述的脊髓灰质炎病毒受体相关蛋白2（Prr 2）的质粒。这种蛋白质，在此命名为HveB，显示介导不能使用HVEM作为共受体的三种突变型HSV-I毒株的进入，但不介导野生型HSV-I毒株的进入。HveB还介导HSV-2和伪狂犬病病毒的进入，但不介导牛疱疹病毒1型。HveB在一些人神经元细胞系、成纤维细胞、角质形成细胞和原代活化T淋巴细胞中表达。HveB特异性抗体阻断表达HveB的CHO细胞和人成纤维细胞株HEL 299的感染。HSV-I和HSV-2株利用HveB进入的能力的差异应影响可被感染的细胞类型，从而部分解释血清型和株系在组织嗜性和致病性方面的差异。(C)北京：科学出版社.
Certain mutant strains of herpes simplex virus type 1 (HSV-1) are unable to infect cells in which entry is dependent on HVEM, the previously described herpesvirus entry mediator designated here as herpesvirus entry protein A (HveA). These mutant viruses can infect other cells where entry is apparently dependent on other co-receptors. The mutant virus HSV-1(KOS)Rid1 was used to screen a human cDNA expression library for ability of transfected plasmids to convert resistant Chinese hamster ovary cells to susceptibility to virus entry. A plasmid expressing the previously described poliovirus receptor-related protein 2 (Prr2) was isolated on the basis of this activity. This protein, designated here as HveB, was shown to mediate the entry of three mutant HSV-I strains that cannot use HVEM as co-receptor, but not wild-type HSV-I strains. HveB also mediated the entry of HSV-2 and pseudorabies virus but not bovine herpesvirus type 1. HveB was expressed in some human neuronal cell lines, fibroblastic cells, keratinocytes, and primary activated T lymphocytes. Antibodies specific for HveB blocked infection of HveB-expressing CHO cells and a human fibroblastic cell strain HEL299. Differences in ability of HSV-I and HSV-2 strains to use HveB for entry should influence the types of cells that can be infected and thereby account in part for serotype and strain differences in tissue tropism and pathogenicity. (C) 1998 Academic Press.