Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS)
Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS)
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DOI：
10.1039/c0lc00403k
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Lee, Luke P.
中科院分区：
文献类型：
--
作者：
Dimov, Ivan K.;Basabe-Desmonts, Lourdes;Lee, Luke P.
我们提出了一种自供电的集成微流体血液分析系统（SIMBAS），不需要任何外部连接，系绳或管道来提供和分析原始全血样品。SIMBAS仅要求用户将5 μ L全血液滴放置在装置的入口处，于是独立的SIMBAS执行红细胞和白色细胞的芯片上去除，而无需外部阀门或泵送机构，随后是含血小板血浆中的分析物检测。在10分钟内进行5次完整的生物素-链霉亲和素样品-应答测定;检测限为1.5 pM。红细胞和白色血细胞通过将它们捕获在整体沟槽结构中而被去除。模拟和实验数据表明，99.9%至100%的血细胞保留在被动结构。SIMBAS的设计指导原则是在不牺牲快速完整生物测定有效性的情况下集成最少数量的组件，这是实现即时分子诊断的关键一步。
We present a self-powered integrated microfluidic blood analysis system (SIMBAS) that does not require any external connections, tethers, or tubing to deliver and analyze a raw whole-blood sample. SIMBAS only requires the user to place a 5 mu L droplet of whole-blood at the inlet port of the device, whereupon the stand-alone SIMBAS performs on-chip removal of red and white cells, without external valving or pumping mechanisms, followed by analyte detection in platelet-containing plasma. Five complete biotin-streptavidin sample-to-answer assays are performed in 10 min; the limit of detection is 1.5 pM. Red and white blood cells are removed by trapping them in an integral trench structure. Simulations and experimental data show 99.9% to 100% blood cell retention in the passive structure. Powered by pre-evacuation of its PDMS substrate, SIMBAS' guiding design principle is the integration of the minimal number of components without sacrificing effectiveness in performing rapid complete bioassays, a critical step towards point-of-care molecular diagnostics.