Effects of a surface prereacted glass?ionomer filler coating material on biofilm formation and inhibition of dentin demineralization
Effects of a surface prereacted glass?ionomer filler coating material on biofilm formation and inhibition of dentin demineralization
复制标题
表面预反应玻璃离子填料涂层材料对生物膜形成和抑制牙本质脱矿的影响
DOI：
10.1007/s00784-020-03577-x
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发表时间：
2020
影响因子：
3.4
通讯作者：
Tagami Junji
中科院分区：
文献类型：
--
作者：
Yamamoto Shiori;Sayed Mahmoud;Takahashi Motoi;Matin Khairul;Hiraishi Noriko;Nikaido Toru;Burrow Michael F.;Tagami Junji
目的本研究探讨表面预反应玻璃离子（S-PRG）涂层材料抑制牙本质生物膜形成和脱矿的能力。（1）无涂层（对照），（2）含S-PRG填料的涂层，（3）不含S-PRG填料的牙本质涂层。然后，测量牙本质表面保留生物膜中的细菌和水不溶性葡聚糖的数量。对于脱矿抑制试验，将样品脱矿5天，然后切成两半，并在共聚焦激光扫描显微镜（CLSM）下观察。单因素方差分析和Tukey的HSD用于statistical analysis.ResultsThe估计的平均表面粗糙度为标本在S-PRG组是统计学显着高于两个nonS-PRG和对照组标本的估计。细菌和水不溶性葡聚糖/mm 2的数量表明，与对照组和非S-PRG组相比，S-PRG组可防止生物膜形成和细菌粘附到牙本质表面。结论在牙本质表面涂敷S-PRG屏障涂层可以抑制生物膜的形成，保护牙本质表面不发生脱矿作用，其临床意义含S-PRG填料的涂层材料可能通过抑制生物膜的形成，促进矿化，减少致龋菌的酸性侵袭而起到防龋作用。
ObjectivesThis study investigated the ability of a surface prereacted glass–ionomer (S-PRG) coating material to inhibit the biofilm formation and demineralization of dentin.Methods and materialsDentin specimens were randomly divided into three groups: (1) no coating (control), (2) S-PRG filler-containing coat, and (3) a nonS-PRG filler–containing coat.Streptococcus mutansbiofilms were grown on the dentin surfaces in a microcosm for 20 h. Then, the quantity of bacteria and water-insoluble glucan in the retained biofilm on the dentin surface were measured. Regarding demineralization inhibition test, specimens were demineralized for 5 days then sectioned into halves and observed under confocal laser scanning microscope (CLSM). One-way ANOVA and Tukey’s HSD were used for statistical analysis.ResultsThe estimated mean surface roughness for specimens in the S-PRG group was statistically significantly higher than the estimates for both the nonS-PRG and the control group specimens. The quantity of bacteria and water-insoluble glucan/mm2revealed that the S-PRG group prevented biofilm formation and bacterial adhesion to the dentin surface compared with the control and nonS-PRG groups. The S-PRG group recorded the highest acid-resistance ability with no surface loss.ConclusionApplication of S-PRG barrier coat on dentin surfaces can inhibit biofilm formation as well as protecting the dentin surface against demineralization.Clinical significanceCoating material containing S-PRG fillers might be used for caries prevention, through inhibiting biofilm formation, enhancing mineralization, and reducing acidic attack by cariogenic bacteria.