Potential applications of polyoxometalates for the discrimination of human papillomavirus in different subtypes
Potential applications of polyoxometalates for the discrimination of human papillomavirus in different subtypes
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多金属氧酸盐在区分人乳头瘤病毒不同亚型方面的潜在应用
DOI：
10.1039/c6dt02186g
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发表时间：
2016
影响因子：
4
通讯作者：
Lixin Wu
中科院分区：
文献类型：
--
作者：
Teng Zhang;Ding-Yi Fu;Yuqing Wu;Yizhan Wang;Lixin Wu
富含精氨酸/赖氨酸的阳离子多肽对含Eu的多金属氧酸盐(POM)EuSiWMo的结合诱导发光增强为检测人乳头瘤病毒(HPV)衣壳蛋白提供了一个平台。然而，它们之间强烈的结合亲和力使得很难区分不同亚型的HPV多肽。因此，本研究采用了几种监测POM-多肽结合亲和力的策略，最终实现了对不同亚型HPV衣壳蛋白代表性多肽的区分。结果表明，具有9个负电荷的含Eu的POM EuW10足以根据特定的序列和基本电荷差异来区分不同亚型的HPV多肽。利用时间分辨荧光光谱和等温滴定量热法(ITC)在亚分子水平上探讨了它们之间的区别机理，揭示了驱动力和结合模型。因此，本研究报道了一种简单、低成本、高效的荧光增强方法来区分不同亚型HPV衣壳蛋白的多肽，这一方法经过进一步的优化是可能的。
The binding-induced luminescence enhancement of an Eu-containing polyoxometalate (POM), EuSiWMo, by the arginin/lysine-rich cationic peptides supplied a platform to detect the capsid proteins of human papillomaviruses (HPVs). However, the strong binding affinity between them makes it very difficult to be differentiated among peptides from different subtypes of HPVs. Therefore, several strategies to monitor the binding affinity of POM-peptide are performed and finally the discriminations on representative peptides from different subtypes of HPV capsid proteins are achieved in the present study. The results show that an Eu-containing POM, EuW10, with nine negative charges is sufficient to discriminate different subtypes of HPV peptides based on the specific sequence and basic charge differences. The discrimination mechanisms between them explored at sub-molecular level using time-resolved fluorescence spectra and isotherm titration calorimetric (ITC) reveal both the driving force and binding model accordingly. Therefore, this study reports a simple, low-cost and efficient fluorescence enhanced method to discriminate the peptides from different subtypes of HPV capsid proteins, which would be possible after further optimization.