EFFICIENCY OF ANTIGEN PRESENTATION TO T-CELL CLONES BY (B-CELLXB-CELL LYMPHOMA) HYBRIDOMAS CORRELATES QUANTITATIVELY WITH CELL-SURFACE IA-ANTIGEN EXPRESSION
EFFICIENCY OF ANTIGEN PRESENTATION TO T-CELL CLONES BY (B-CELLXB-CELL LYMPHOMA) HYBRIDOMAS CORRELATES QUANTITATIVELY WITH CELL-SURFACE IA-ANTIGEN EXPRESSION
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DOI：
10.1002/eji.1830140908
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
PIERRES, M
中科院分区：
文献类型：
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作者：
BEKKHOUCHA, F;NAQUET, P;PIERRES, M
使用一系列 B 细胞杂交瘤作为模型系统来定量评估 Ia 分子在抗原呈递给同种异体或可溶性抗原反应性 T 细胞克隆中的作用。这些杂交细胞系是通过 HGPRT-BALB/c B [小鼠] 细胞淋巴瘤 M12.4.1 和 LPS [脂多糖] 刺激的 B10.BR (H-2k) 小鼠脾细胞融合而建立的。适当的抗-Ia单克隆抗体的定量细胞吸收和流式细胞荧光分析表明，所检查的B细胞杂交瘤组成型表达大量表面I-Ak或I-Ek分子，其数量级变化为1-5。这种定量差异与 B 细胞杂交瘤响应 E.beta.k 同种异体决定簇或 I-Ak 限制元件中呈递的聚 (Glu60Ala30Tyr10) 激活 T 细胞克隆增殖和/或产生白细胞介素 2 的能力，以及抑制抗原呈递给 T 细胞克隆所需的单克隆抗 I-Ak 抗体的量精确相关。这些数据的可能含义在当前抗原呈递细胞调节 Ia 抗原表达的模型的背景下进行了讨论。
A series of B cell hybridomas was used as a model system to assess quantitatively the role of Ia molecules in antigen presentation to allo- or soluble antigen-reactive T cell clones. These hybrid cell lines were established by fusion between the HGPRT- BALB/c B [mouse] cell lymphoma M12.4.1 and LPS [lipopolysaccharide]-stimulated spleen blasts from B10.BR (H-2k) mice. Quantitative cellular absorption of appropriate anti-Ia monoclonal antibodies and flow cytofluorometric analyses revealed that the B cell hybridomas examined expressed constitutively a number of surface I-Ak or I-Ek molecules that varied in an order of magnitude of 1-5. Such quantitative differences were correlated precisely with the capacity of B cell hybridomas to activate T cell clones to proliferate and/or to produce interleukin 2 in response to E.beta.k allodeterminant or to poly(Glu60Ala30Tyr10) presented in the context of I-Ak restriction element, and the amount of monoclonal anti-I-Ak antibody required to inhibit antigen presentation to T cell clones. The possible implications of these data are discussed in the context of current models of regulation of Ia antigen expression by antigen-presenting cells.