Protocols for in vitro Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and Adipogenic Lineages
Protocols for in vitro Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and Adipogenic Lineages
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DOI：
10.1007/978-1-4939-3584-0_8
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Gnecchi, Massimiliano
中科院分区：
文献类型：
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作者：
Ciuffreda, Maria Chiara;Malpasso, Giuseppe;Gnecchi, Massimiliano
间充质干细胞(MSC)具有高度的可塑性和分化为多种不同细胞类型的潜能；这一特性对细胞治疗和修复生物技术具有重要意义。MSC最初是从骨髓中分离出来的，但在其他组织中也发现了它们，如脂肪组织、脐带血、滑膜、骨骼肌和肺。在体外和体内，MSC可以分化为骨、骨细胞、软骨细胞、脂肪细胞和骨骼肌细胞等多种细胞类型。在过去的二十年里，越来越多的研究证明MSC在治疗神经退行性疾病、脊髓和脑损伤、心血管疾病、糖尿病和骨骼疾病方面具有潜在的治疗潜力。他们的免疫特权档案允许自体和同种异体使用。由于这些原因，MSC的科学吸引力不断上升。目前，MSC的鉴定基于三个主要标准：塑料黏附能力、明确的表位分布以及在体外分化为骨细胞、软骨细胞和脂肪细胞的能力。在这里，我们描述了BM-MSC分化为成骨、成软骨和成脂的标准方案。
Mesenchymal stem cells (MSC) possess high plasticity and the potential to differentiate into several different cell types; this characteristic has implications for cell therapy and reparative biotechnologies. MSC have been originally isolated from the bone marrow (BM-MSC), but they have been found also in other tissues such as adipose tissue, cord blood, synovium, skeletal muscle, and lung. MSC are able to differentiate in vitro and in vivo into several cell types such as bone, osteocytes, chondrocytes, adipocytes, and skeletal myocytes, just to name a few.During the last two decades, an increasing number of studies have proven the therapeutic potential of MSC for the treatment of neurodegenerative diseases, spinal cord and brain injuries, cardiovascular diseases, diabetes mellitus, and diseases of the skeleton. Their immuno-privileged profile allows both autologous and allogeneic use. For all these reasons, the scientific appeal of MSC is constantly on the rise.The identity of MSC is currently based on three main criteria: plastic-adherence capacity, defined epitope profile, and capacity to differentiate in vitro into osteocytes, chondrocytes, and adipocytes. Here, we describe standard protocols for the differentiation of BM-MSC into the osteogenic, chondrogenic, and adipogenic lineages.