Increased salivary microvesicles are associated with the prognosis of patients with oral squamous cell carcinoma
Increased salivary microvesicles are associated with the prognosis of patients with oral squamous cell carcinoma
复制标题
唾液微泡增多与口腔鳞状细胞癌患者的预后相关
DOI：
10.1111/jcmm.14291
复制
发表时间：
2019
影响因子：
5.3
通讯作者：
Chen Gang
中科院分区：
文献类型：
--
作者：
Zhong Wen Qun;Ren Jian Gang;Xiong Xue Peng;Man Qi Wen;Zhang Wei;Gao Lu;Li Chen;Liu Bing;Sun Zhi Jun;Jia Jun;Zhang Wen Feng;Zhao Yi Fang;Chen Gang
微泡（MV）是存在于体液中的细胞衍生的膜囊泡，与恶性肿瘤的发展密切相关。唾液是人体最常见的体液之一，也是MV的重要来源。然而，唾液MV（SMVs）和口腔鳞状细胞癌（OSCC），这是直接浸入唾液环境之间的关联，仍然不清楚。对65例口腔鳞癌、21例口腔溃疡（oral ulcer，OSCC）和42例健康志愿者的SMV进行纯化、定量，并分析其与OSCC患者临床病理特征和预后的关系。结果显示，与健康供体和口腔鳞癌患者相比，口腔鳞癌患者的SMV水平显著升高。SMV水平与淋巴结转移状况、临床分期密切相关。此外，在具有较高病理分级的OSCC患者中，凋亡与非凋亡SMV的比率显著降低。一致地，在凋亡与非凋亡SMV比率较低的患者中观察到较差的总生存期。总之，SMV水平升高与口腔鳞癌患者的临床病理特征和生存率降低相关，这表明SMV是口腔鳞癌恶性进展的潜在生物标志物和/或调节剂。
Microvesicles (MVs), which are cell‐derived membrane vesicles present in body fluids, are closely associated with the development of malignant tumours. Saliva, one of the most versatile body fluids, is an important source of MVs. However, the association between salivary MVs (SMVs) and oral squamous cell carcinoma (OSCC), which is directly immersed in the salivary milieu, remains unclear. SMVs from 65 patients with OSCC, 21 patients with oral ulcer (OU), and 42 healthy donors were purified, quantified and analysed for their correlations with the clinicopathologic features and prognosis of OSCC patients. The results showed that the level of SMVs was significantly elevated in patients with OSCC compared to healthy donors and OU patients. Meanwhile, the level of SMVs showed close correlations with the lymph node status, and the clinical stage of OSCC patients. Additionally, the ratio of apoptotic to non‐apoptotic SMVs was significantly decreased in OSCC patients with higher pathological grade. Consistently, poorer overall survival was observed in patients with lower ratio of apoptotic to non‐apoptotic SMVs. In conclusion, the elevated level of SMVs is associated with clinicopathologic features and decreased survival in patients with OSCC, suggesting that SMVs are a potential biomarker and/or regulator of the malignant progression of OSCC.