Cell effects on the formation of collagen triple helix fibers inside collagen gels or on cell surfaces
Cell effects on the formation of collagen triple helix fibers inside collagen gels or on cell surfaces
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DOI：
10.1038/pj.2015.2
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发表时间：
2015-05
期刊：
影响因子：
2.8
通讯作者：
Chun‐Yen Liu;M. Matsusaki;M. Akashi
中科院分区：
文献类型：
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作者：
Chun‐Yen Liu;M. Matsusaki;M. Akashi
胶原分子广泛应用于组织工程领域，特别是作为细胞培养水凝胶。然而，细胞对胶原蛋白凝胶内胶原纤维形成的影响尚未报道。在这里，我们评估了胶原蛋白水凝胶或细胞表面上的分子水平上的细胞对胶原蛋白三螺旋纤维形成的影响。胶原凝胶的溶胀率随着细胞数量的增加而降低，这很可能是因为细胞表面整合素分子与胶原纤维之间的相互作用。圆二色性和紫外可见光谱分析表明，细胞和胶原分子的聚集，因为它们的强相互作用。X-射线衍射分析表明，细胞并没有影响胶原蛋白凝胶内胶原蛋白纤维的弱分子取向，因为胶原蛋白凝胶通常不具有肌腱中天然胶原蛋白纤维那样致密的分子堆积。扫描电子显微镜显示聚集的亚微米大小的球形胶原蛋白结构，主要围绕细胞内的胶原蛋白凝胶。结果表明，细胞表面和胶原蛋白分子之间的相互作用诱导形成球形结构，而不是微纤维形成，这反过来表明，细胞和胶原蛋白分子之间的相互作用诱导胶原蛋白分子吸附到细胞表面上，并导致在水凝胶中形成球形结构。
Collagen molecules are widely used in the field of tissue engineering, especially as cell culture hydrogels. However, the cellular effects on collagen fiber formation inside collagen gels have not been reported. Here, we evaluated the effect of cells on collagen triple helix fiber formation at a molecular level in collagen hydrogels or on cell surfaces. Swelling ratios of collagen gels decreased with increasing cell numbers, most likely because of interactions between cell surface integrin molecules and collagen fibers. Circular dichroism and ultraviolet–visible spectroscopic analyses revealed aggregations of cells and collagen molecules because of their strong interaction. X-ray diffraction analyses suggested that cells did not affect the weak molecular orientation of collagen fibers inside collagen gels because the collagen gels usually do not have as dense molecular packing as natural collagen fibers in tendons. Scanning electron microscopy showed aggregations of submicrometer-sized spherical collagen structures mainly around cells inside collagen gels. The results suggest that interactions between cell surfaces and collagen molecules induce the formation of spherical constructs rather than microfiber formation, which in turn indicates that interactions between cells and collagen molecules induce the adsorption of collagen molecules onto cell surfaces and lead to the formation of spherical constructs in hydrogels.