Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus
Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus
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DOI：
10.1126/science.1090956
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发表时间：
2003-11-28
期刊：
影响因子：
56.9
通讯作者：
Tenover, FC
中科院分区：
文献类型：
--
作者：
Weigel, LM;Clewell, DB;Tenover, FC
万古霉素通常用于治疗严重感染，包括由多重耐药金黄色葡萄球菌引起的感染。临床分离的S.在2002年6月分离出对万古霉素具有高水平抗性的金黄色葡萄球菌（最小抑菌浓度= 1024 μ g/ml）。该分离物含有一个57.9-内切酶多抗性接合质粒，其中整合了Tn 1546（vanA）。质粒上的其他元件编码对甲氧苄啶（dfrA）、β-内酰胺类（blaZ）、氨基糖苷类（aacA-aphD）和消毒剂（qacC）的抗性。遗传分析表明，万古霉素耐药性转移到耐甲氧西林的S。金黄色葡萄球菌发生在体内的种间转移的Tn 1546从粪肠球菌的共分离。
Vancomycin is usually reserved for treatment of serious infections, including those caused by multidrug-resistant Staphylococcus aureus. A clinical isolate of S. aureus with high-level resistance to vancomycin ( minimal inhibitory concentration = 1024 mug/ml) was isolated in June 2002. This isolate harbored a 57.9-kilobase multiresistance conjugative plasmid within which Tn1546 (vanA) was integrated. Additional elements on the plasmid encoded resistance to trimethoprim (dfrA), beta-lactams (blaZ), aminoglycosides (aacA-aphD), and disinfectants ( qacC). Genetic analyses suggest that the long-anticipated transfer of vancomycin resistance to a methicillin-resistant S. aureus occurred in vivo by interspecies transfer of Tn1546 from a co-isolate of Enterococcus faecalis.