Application of molecularly imprinted polymers for the analysis of polycyclic aromatic hydrocarbons in lipid matrix-based biological samples
Application of molecularly imprinted polymers for the analysis of polycyclic aromatic hydrocarbons in lipid matrix-based biological samples
复制标题
分子印迹聚合物在脂质基质生物样品中多环芳烃分析中的应用
DOI：
10.1007/s00216-017-0646-6
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发表时间：
2017-11-01
影响因子：
4.3
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Sun, Ying;Pang, Yiming;Wang, Bin
高精度分析生物样品中的多环芳烃是非常必要的。我们研究了市售的分子印迹聚合物（MIP）用于分析各种生物样品（即，黄油、花生油、猪肚和人脐带），并将该方法与常用的凝胶渗透色谱（GPC）进行比较。在用MIP或GPC柱初步净化后，用中性氧化铝柱进一步净化萃取物。然后，我们用气相色谱-质谱法测定了16个PAH同系物。MIP方法允许PAH回收水平与GPC方法测量的那些一样高。超过95%的黄油，花生油和五花肉的粗脂肪被去除，尽管只有50%的人脐带被去除。四种样品经MIP方法净化后的最终提取物的扫描离子色谱图的响应与溶剂空白相似，略优于GPC净化后的结果。此外，MIP操作空白中的PAH浓度通常低于GPC操作空白。这些结果表明，分子印迹聚合物可应用于各种脂质基质生物样品中多环芳烃的分析。图示摘要分子印迹聚合物色谱柱示意图及各种生物样品中多环芳烃和粗脂肪的洗脱曲线
High-accuracy analyses of polycyclic aromatic hydrocarbons (PAHs) in lipid matrix-based biological samples are highly necessary. We investigated the cleanup performance of the commercially available molecularly imprinted polymers (MIPs) to analyze PAHs in various biological samples (i.e., butter, peanut oil, pork belly, and human umbilical cord), and compared this method with the commonly used gel permeation chromatography (GPC). After primary cleanup with a MIP or GPC column, the extracts were further cleaned with a neutral aluminum oxide column. Then, we measured 16 PAH congeners using gas chromatography-mass spectrometry. The MIP method allowed PAH recovery levels as high as those measured with the GPC method to be obtained. More than 95% of the crude fats of butter, peanut oil, and pork belly were removed, although only ~ 50% were removed for human umbilical cord. The scan-mode ion chromatograms of the final extracts cleaned with the MIP method had responses similar to solvent blank for the four types of samples, which were slightly better than those cleaned with GPC. Moreover, the PAH concentrations in the MIP operation blank were generally lower than those of GPC operation blanks. These results indicated that MIP could be applied to the analysis of PAHs in various lipid matrix-based biological samples.Graphical abstractThe schematic diagram of a molecularly imprinted polymer column and the elution curves of polycyclic aromatic hydrocarbons and crude fats of various biological samples