IGF-I induces collagen and IGFBP-5 mRNA in rat intestinal smooth muscle.
IGF-I induces collagen and IGFBP-5 mRNA in rat intestinal smooth muscle.
复制标题
IGF-I 在大鼠肠平滑肌中诱导胶原蛋白和 IGFBP-5 mRNA。
DOI：
10.1152/ajpgi.1997.273.4.g875
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bitar,KN
中科院分区：
文献类型：
--
作者：
Zimmermann,EM;Li,L;Hou,YT;Cannon,M;Christman,GM;Bitar,KN
通过 Northern 分析和原位杂交，研究了肽聚糖-多糖小肠结肠炎大鼠肠道中的胰岛素样生长因子 (IGF) 结合蛋白 5 (IGFBP-5) mRNA。与对照组相比，发炎大鼠结肠中 IGFBP-5 mRNA 增加 2.4 ± 0.5 倍，并且在平滑肌中高表达。利用培养的大鼠肠平滑肌细胞研究IGFBP-5和I型胶原合成的调节。 IGF-I (100 ng/ml) 使培养的平滑肌细胞中的 IGFBP-5 mRNA (1.9 ± 0.1 倍) 和胶原蛋白 α1(I) mRNA (1.6 ± 0.2 倍) 增加。通过Western配体印迹和免疫印迹，IGF-I诱导条件培养基中IGFBP-5的剂量和时间依赖性增加。 IGF-I 不影响转录阻断后 IGFBP-5 mRNA 的衰减率。放线菌酮消除了 IGFBP-5 mRNA。总之，IGFBP-5 mRNA 由肠道平滑肌表达，并在慢性炎症过程中增加。 IGF-I 增加肠道平滑肌细胞中的 IGFBP-5 和胶原蛋白 mRNA。
Insulin-like growth factor (IGF) binding protein 5 (IGFBP-5) mRNA was studied in intestines of rats with peptidoglycan-polysaccharide enterocolitis by Northern analysis and in situ hybridization. IGFBP-5 mRNA was increased 2.4 ± 0.5-fold in inflamed rat colon compared with controls and was highly expressed in smooth muscle. Cultured rat intestinal smooth muscle cells were used to study the regulation of IGFBP-5 and type I collagen synthesis. IGF-I (100 ng/ml) increased IGFBP-5 mRNA (1.9 ± 0.1-fold) and collagen type α1(I) mRNA (1.6 ± 0.2-fold) in cultured smooth muscle cells. IGF-I induced a dose- and time-dependent increase in IGFBP-5 in conditioned medium by Western ligand blot and by immunoblot. IGF-I did not affect the IGFBP-5 mRNA decay rate after transcriptional blockade. Cycloheximide abolished IGFBP-5 mRNA. In conclusion, IGFBP-5 mRNA is expressed by intestinal smooth muscle and is increased during chronic inflammation. IGF-I increases IGFBP-5 and collagen mRNAs in intestinal smooth muscle cells.