The slit receptor Rig-1/Robo3 controls midline crossing by hindbrain precerebellar neurons and axons
The slit receptor Rig-1/Robo3 controls midline crossing by hindbrain precerebellar neurons and axons
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DOI：
10.1016/j.neuron.2004.06.018
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发表时间：
2004-07-08
期刊：
影响因子：
16.2
通讯作者：
Chédotal, A
中科院分区：
文献类型：
--
作者：
Marillat, V;Sabatier, C;Chédotal, A
在发育过程中，小脑前神经元从菱形唇向底板背腹侧迁移。其中一些神经元穿过中线，而另一些则停止。我们已经确定了狭缝受体Rig-1/Robo 3在指导这一过程中的作用。在其切线迁移，所有主要的后脑小脑前核神经元表达高水平的Rig-1 mRNA。Rig-1的表达随着其前导过程穿过底板而迅速下调。有趣的是，大多数小脑前核在Rig-1缺陷小鼠中不能正常发育，因为它们不能穿过中线。此外，下橄榄核神经元，通常发送轴突到对侧小脑，项目同侧在Rig-1突变小鼠。类似地，外侧网状核和基底脑桥的神经元不能迁移穿过底板，而是保持同侧。这些结果表明，Rig-1控制小脑前神经元细胞体及其轴突跨越中线的能力。
During development, precerebellar neurons migrate dorsoventrally from the rhombic lip to the floor plate. Some of these neurons cross the midline while others stop. We have identified a role for the slit receptor Rig-1/Robo3 in directing this process. During their tangential migration, neurons of all major hindbrain precerebellar nuclei express high levels of Rig-1 mRNA. Rig-1 expression is rapidly downregulated as their leading process crosses the floor plate. Interestingly, most precerebellar nuclei do not develop normally in Rig-1-deficient mice, as they fail to cross the midline. In addition, inferior olivary neurons, which normally send axons into the contralateral cerebellum, project ipsilaterally in Rig-1 mutant mice. Similarly, neurons of the lateral reticular nucleus and basilar pons are unable to migrate across the floor plate and instead remain ipsilateral. These results demonstrate that Rig-1 controls the ability of both precerebellar neuron cell bodies and their axons to cross the midline.