Location and motion of free cholesterol molecules in high density lipoprotein.
Location and motion of free cholesterol molecules in high density lipoprotein.
复制标题
高密度脂蛋白中游离胆固醇分子的位置和运动。
DOI：
10.1016/0006-291x(81)90719-1
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发表时间：
1981
影响因子：
3.1
通讯作者：
Phillips,MC
中科院分区：
文献类型：
--
作者：
Lund-Katz,S;Phillips,MC
用4-[13 C]-胆固醇取代游离胆固醇分子重建人高密度脂蛋白（HDL 3）。获得了90 MHz [13 C]-NMR谱，并可分辨化学位移为41.73和42.20 ppm的两个胆固醇共振。前一种信号来自与磷脂结合的胆固醇分子的C-4原子，位于HDL 3颗粒的表面，而后一种共振是由于与胆固醇酯和甘油三酯分子结合的胆固醇分子在核心。不含任何胆固醇酯或甘油三酯的HDL 3重建在41.73 ppm处产生单一共振，表明所有游离胆固醇分子都在表面。正常HDL 3中存在的60%游离胆固醇分子位于表面周围的磷脂单层中，与位于液核中的其余40%相比，它们在那里进行相对受限的运动。游离胆固醇分子可以在10 ms-700 s的时间尺度内在两个池之间平衡。
Human high density lipoprotein (HDL3) was reconstituted with the free cholesterol molecules replaced with 4-[13C]-cholesterol. 90 MHz [13C]-NMR spectra were obtained and two cholesterol resonances at chemical shifts of 41.73 and 42.20 ppm could be resolved. The former signal arises from the C-4 atom of cholesterol molecules associated with phospholipids and located in the surface of the HDL3particle while the latter resonance is due to cholesterol molecules associated with cholesterol ester and triglyceride molecules in the core. HDL3reconstituted without any cholesterol ester or triglyceride gave a single resonance at 41.73 ppm indicating that all the free cholesterol molecules are in the surface. 60% of the free cholesterol molecules present in normal HDL3are in the phospholipid monolayer around the surface where they undergo relatively restricted motion compared to the remaining 40% situated in the liquid core. The free cholesterol molecules can equilibrate between the two pools in the timescale 10ms–700s.