ALGINATE FROM PSEUDOMONAS-FLUORESCENS AND P-PUTIDA - PRODUCTION AND PROPERTIES
ALGINATE FROM PSEUDOMONAS-FLUORESCENS AND P-PUTIDA - PRODUCTION AND PROPERTIES
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DOI：
10.1099/13500872-140-5-1125
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发表时间：
1994-05-01
期刊：
影响因子：
2.8
通讯作者：
SUTHERLAND, IW
中科院分区：
文献类型：
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作者：
CONTI, E;FLAIBANI, A;SUTHERLAND, IW
以葡萄糖和果糖为碳源，采用分批培养法制备了荧光假单胞菌和恶臭假单胞菌合成的类藻酸盐类多糖。尽管两种底物的分解代谢方法和藻酸盐前体的合成方法不同，但两株菌株产生的多糖在甘露糖酸和古鲁糖酸的组成以及D-甘露糖酸二聚体的出现频率上是一致的。所有制剂都缺少L-古糖醛酸的同源寡聚序列，并且高度乙酰化(12-21%)。在所测试的所有培养条件下，多糖的产生与生长相关，并在生长48h后达到最大M(R)；较老的培养物含有逐渐降低的M(R)。这归因于藻酸盐裂解酶的降解活性，这种酶在两个物种的细胞内都被检测到，并可能通过细胞裂解释放。培养72小时，恶臭假单胞菌的M(R)仅为34000-38500，而以果糖为底物的荧光假单胞菌的M(R)为300000，葡萄糖培养的恶臭假单胞菌的M(R)为72000。
The alginate-like polysaccharides synthesized by Pseudomonas fluorescens and Pseudomonas putida have been prepared from batch cultures grown with glucose and fructose as carbon substrates. Despite the different methods of catabolism of the two substrates and synthesis of the alginate precursors, both strains produced polysaccharides which were consistent in their composition of mannuronic and guluronic acids and in the frequency of occurrence of dimers of D-mannuronic acid. All preparations lacked homooligomeric sequences of L-guluronic acid and were highly acetylated (12-21%). In all the culture conditions tested, polysaccharide production was growth-associated and maximum M(r) was obtained after 48 h growth; older cultures contained material of progressively lower M(r). This was ascribed to the degradative activity of alginate lyases which were detected intracellularly in both species and are presumably released by cell lysis. At 72 h, alginate from P. putida grown on either substrate had an M(r) of only 34000-38500, whereas the product from P. fluorescens grown on fructose had an M(r) of 300000 and that from glucose-grown cultures an M(r) of 72000.