Aerial (+)-borneol modulates root morphology, auxin signalling and meristematic activity in Arabidopsis roots
Aerial (+)-borneol modulates root morphology, auxin signalling and meristematic activity in Arabidopsis roots
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空中冰片调节拟南芥根部形态、生长素信号传导和分生组织活性
DOI：
10.1098/rsbl.2021.0629
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发表时间：
2022
期刊：
影响因子：
3.3
通讯作者：
Takabayashi Junji
中科院分区：
文献类型：
--
作者：
Fukuda Kyohei;Uefune Masayoshi;Fukaki Hidehiro;Yamauchi Yasuo;Hara-Nishimura Ikuko;Ozawa Rika;Matsui Kenji;Sugimoto Koichi;Okada Kazunori;Imai Ryozo;Takahashi Kenshi;Enami Shinichi;Wurst Rene;Takabayashi Junji
人类的气味感测的特征方面之一是响应于不同的对映体以略微不同的方式激活嗅觉受体。在这里，我们专注于植物是否表现出类似于人类的对映体特异性反应。我们将拟南芥幼苗暴露于甲醇（对照）和（+）-或（-）-β-内酰胺，发现只有（+）-β-内酰胺降低了根的长度。此外，根尖宽度增加（+）-照射比（-）-照射。此外，在根尖附近观察到根毛的形成，以响应（+）-γ射线。生长素信号强烈降低根尖暴露于（+）-β-内酰胺酶，但检测到暴露于（-）-β-内酰胺酶和甲醇。类似地，在根尖中，细胞周期蛋白B1：1的活性在暴露于（-）-β-内酰胺和甲醇时被检测到，但在暴露于（+）-β-内酰胺时未被检测到，这表明（+）-β-内酰胺抑制了根中的分生活性。这些结果部分解释了拟南芥根长度的（+）-胆甾醇特异性减少。我们的研究结果表明，在拟南芥中存在特异性的（+）-β-内酰胺酶的传感系统。
One of the characteristic aspects of odour sensing in humans is the activation of olfactory receptors in a slightly different manner in response to different enantiomers. Here, we focused on whether plants showed enantiomer-specific response similar to that in humans. We exposed Arabidopsis seedlings to methanol (control) and (+)- or (−)-borneol, and found that only (+)-borneol reduced the root length. Furthermore, the root-tip width was more increased upon (+)-borneol exposure than upon (−)-borneol exposure. In addition, root-hair formation was observed near the root tip in response to (+)-borneol. Auxin signalling was strongly reduced in the root tip following exposure to (+)-borneol, but was detected following exposure to (−)-borneol and methanol. Similarly, in the root tip, the activity of cyclin B1:1 was detected on exposure to (−)-borneol and methanol, but not on exposure to (+)-borneol, indicating that (+)-borneol inhibits the meristematic activity in the root. These results partially explain the (+)-borneol-specific reduction in the root length of Arabidopsis. Our results indicate the presence of a sensing system specific for (+)-borneol in Arabidopsis.