Applications of functional protein microarrays in basic and clinical research.
Applications of functional protein microarrays in basic and clinical research.
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DOI：
10.1016/b978-0-12-394395-8.00004-9
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发表时间：
2012
影响因子：
--
通讯作者：
Qian, Jiang
中科院分区：
文献类型：
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作者：
Zhu, Heng;Qian, Jiang
蛋白质微阵列技术为以高度并行和高通量的方式表征数十万种蛋白质提供了一个通用的平台。它被视为一种克服DNA微阵列局限性的新工具。根据其应用，蛋白质微阵列主要分为两大类：分析型和功能性蛋白质微阵列。此外，组织或细胞裂解产物也可以直接在载玻片上斑点，形成所谓的“反相”蛋白质微阵列。在过去的十年里，功能蛋白质微阵列在研究蛋白质功能以及构建网络和通路方面的应用尤其蓬勃发展。在这一章中，我们将回顾蛋白质微阵列技术的最新进展，然后提供一系列的例子来说明蛋白质微阵列在基础和临床研究中的威力和多功能性。作为一个强大的技术平台，如果蛋白质微阵列在未来十年成为蛋白质组和诊断领域的领先技术之一，也就不足为奇了。
The protein microarray technology provides a versatile platform for characterization of hundreds of thousands of proteins in a highly parallel and high-throughput manner. It is viewed as a new tool that overcomes the limitation of DNA microarrays. On the basis of its application, protein microarrays fall into two major classes: analytical and functional protein microarrays. In addition, tissue or cell lysates can also be directly spotted on a slide to form the so-called “reverse-phase” protein microarray. In the last decade, applications of functional protein microarrays in particular have flourished in studying protein function and construction of networks and pathways. In this chapter, we will review the recent advancements in the protein microarray technology, followed by presenting a series of examples to illustrate the power and versatility of protein microarrays in both basic and clinical research. As a powerful technology platform, it would not be surprising if protein microarrays will become one of the leading technologies in proteomic and diagnostic fields in the next decade.