In vitro evaluation of the root canal cleaning ability of plant extracts and their antimicrobial action
In vitro evaluation of the root canal cleaning ability of plant extracts and their antimicrobial action
复制标题
DOI：
10.1590/s1806-83242012000300006
复制
发表时间：
2012-06-01
影响因子：
2.5
通讯作者：
Carvalho, Rejane Andrade de
中科院分区：
文献类型：
--
作者：
Costa, Edja Maria Melo de Brito;Evangelista, Augusto Pierry de Araújo;Carvalho, Rejane Andrade de
这项体外研究评价了冲洗液中使用的植物提取物的抗菌活性和根管清洁能力。采用琼脂孔扩散法测定了石杉和木姜子水醇提取物、2.5%次氯酸钠和0.12%洗必泰（阳性对照）对粪肠球菌（Enterococcus faecalis）的抗菌活性。用扫描电镜观察根管清洁度。将21颗单根牙根据所用冲洗液分为3组：1）50%阿罗贝拉-达普拉亚; 2）50%奎沙贝拉; 3）2.5%次氯酸钠+17%EDTA的组合。所有测试的溶液均表现出对粪肠球菌的抗微生物活性。SEM分析显示，在三个组中，分别观察到冠状面和根尖三分之一的表面清洁程度较高和较低，其中quixabeira在去除根尖三分之一的玷污层方面表现出最大的效率。所有供试制剂对大肠杆菌均有抗菌活性。粪便。然而，没有一种方法能够完全去除根管不同三分之一处牙本质表面的玷污层。结果表明，所分析的植物提取物可能代表了一个新的治疗选择的替代剂名单可用于牙髓治疗。
This in vitro study evaluated both the antimicrobial activity and the root canal cleaning ability of plant extracts used in irrigation solutions. The antimicrobial activities of the aroeira-da-praia (Schinus terebintifolius Raddi) and the quixabeira (Syderoxylum obtusifolium Roem & Schult) hydroalcoholic extracts, of 2.5% sodium hypochlorite (NaOCl) and of 0.12% chlorhexidine (positive control) against Enterococcus faecalis were tested with the agar well diffusion method. The level of root canal cleanliness was examined by scanning electron microscopy (SEM). Twenty one single-rooted human teeth were divided into three groups according to the irrigation solution applied: 1) 50% aroeira-dapraia; 2) 50% quixabeira and 3) a combination of 2.5% sodium hypochlorite + 17% EDTA. All solutions tested demonstrated antimicrobial activity against Enterococcus faecalis. The SEM analysis revealed that higher and lower degrees of surface cleaning were observed, in the three groups, respectively for the coronal and apical thirds, in that quixabeira showed the greatest efficiency in removing the smear layer in the apical third. All the agents tested presented antimicrobial activity against E. faecalis. None, however, was able to completely remove the smear layer of the dentinal surface in the different thirds of the root canal. The results suggest that the analyzed plant extracts may represent a new therapeutic option in the list of alternative agents available for endodontic treatment.