Serum opsonic activity in rodent malaria: functional and immunochemical characteristics in vitro.
Serum opsonic activity in rodent malaria: functional and immunochemical characteristics in vitro.
复制标题
啮齿动物疟疾的血清调理活性：体外功能和免疫化学特征。
DOI：
10.4049/jimmunol.123.6.2582
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发表时间：
1979
影响因子：
4.4
通讯作者：
T. W. Simpson
中科院分区：
文献类型：
--
作者：
K. Hunter;J. Winkelstein;T. W. Simpson
本研究检查了啮齿动物疟疾血清调理活性的功能和免疫化学特征。在聚蔗糖密度梯度上分离富含裂殖体和晚期滋养体的伯氏疟原虫感染的红细胞（IRBC）群体，并用于由大鼠腹膜巨噬细胞的血清和单层培养物组成的体外吞噬系统。超免疫血清比非免疫血清更大程度地增强 IRBC 的吞噬作用。当 IRBC 或巨噬细胞与血清预孵育时，吞噬促进因子以血清调理素特有的方式作用于 IRBC 而不是巨噬细胞。调理活性对于 IRBC 是特异性的，因为未感染的红细胞很少被吞噬并且无法从血清中吸收调理活性。超免疫和非免疫血清的调理活性都是热稳定的，并且不受已知的灭活或抑制补体的试剂（眼镜蛇毒因子和乙二胺四乙酸）的影响。最后，在从超免疫和非免疫血清中分离的纯化 IgG 制剂中鉴定了调理活性。
The functional and immunochemical characteristics of serum opsonic activity in rodent malaria were examined in the present study. Schizont- and late trophozoite-enriched populations of Plasmodium berghei-infected red blood cells (IRBC) were isolated on a Ficoll density-gradient and used in an in vitro phagocytosis system composed of serum and monolayer cultures of rat peritoneal macrophages. Hyperimmune serum augmented the phagocytosis of IRBC to a greater degree than did nonimmune serum. When either IRBC or macrophages were pre-incubated with serum, the phagocytosis-promoting factors acted on the IRBC rather than on the macrophages in a manner characteristic of serum opsonins. The opsonic activity was specific for IRBC since noninfected red blood cells were rarely phagocytized and were unable to absorb opsonic activity from serum. The opsonic activity of both hyperimmune and nonimmune sera was heat stable, and unaffected by agents known to inactivate or inhibit complement (cobra venom factor and ethylenediaminetetraacetic acid). Finally, the opsonic activity was identified in preparations of purified IgG isolated from both hyperimmune and nonimmune sera.