Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine
Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine
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DOI：
10.1016/0264-410x(96)00020-5
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发表时间：
1996-07-01
期刊：
影响因子：
5.5
通讯作者：
Poolman, J
中科院分区：
文献类型：
--
作者：
Claassen, I;Meylis, J;Poolman, J
从两种基因工程菌株制备实验血清群B脑膜炎球菌疫苗;每种菌株表达三种不同的1类外膜蛋白（OMP）（PorA）。两株病毒分别表达P1.7，16，P1.5，2; P1.19，15和P1.5（c），10; P1.12，13;P1.7（h），4亚型。通过脱氧胆酸盐提取从这些菌株制备外膜囊泡（OMV），与作为佐剂的磷酸铝混合并配制成最终疫苗。1类OMP占疫苗中蛋白质的约90%。该疫苗被发现对人类使用是安全的，并在小鼠中诱导针对六种野生型菌株中的五种的杀菌免疫应答，所述野生型菌株充当各种por A基因的供体。版权所有（C）1996 Elsevier Science Ltd.
An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16,P1.5,2;P1.19,15 and P1.5(c),10;P1.12,13;P1.7(h),4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various por A genes. Copyright (C) 1996 Elsevier Science Ltd.