Role of CTGF/CCN2 in reparative dentinogenesis in human dental pulp
Role of CTGF/CCN2 in reparative dentinogenesis in human dental pulp
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DOI：
10.2334/josnusd.54.47
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发表时间：
2012-03-01
影响因子：
1.9
通讯作者：
Matsushima, Kiyoshi
中科院分区：
文献类型：
--
作者：
Muromachi, Koichiro;Kamio, Naoto;Matsushima, Kiyoshi
结缔组织生长因子/CCN家族2 （CTGF/CCN2）被认为参与了牙齿的发育。迄今为止，CTGF/CCN2在修复性牙本质发生中的表达和作用尚不清楚。我们前期研究发现基质金属蛋白酶-3 （matrix metalloproteinase-3, MMP-3）在人牙髓细胞中通过CTGF/CCN2的表达和分泌刺激细胞迁移，且这一过程依赖于动力蛋白相关的内吞作用，不依赖于蛋白酶活性。本研究的目的是检测CTGF/CCN2在人龋齿修复牙本质中的表达，并探讨CTGF/CCN2对培养的人牙髓细胞矿化的影响。CTGF/CCN2在健康牙齿牙本质-牙髓交界处附近的成牙细胞中表达极低，而在龋附近修复性牙本质周围的成牙细胞样细胞中表达强烈。在人牙髓细胞中，CTGF/CCN2促进矿化，但不能诱导增殖，提示该分子具有诱导人牙髓细胞分化的能力。综上所述，这些数据表明CTGF/CCN2可能通过在人类龋齿中形成硬组织参与修复性牙本质形成。
Connective tissue growth factor/ CCN family 2 (CTGF/CCN2) has been considered to participate in tooth development. To date, the expression and role of CTGF/CCN2 in reparative dentinogenesis have been unclear. Our previous study revealed that matrix metalloproteinase-3 (MMP-3) stimulates cell migration via CTGF/CCN2 expression and secretion in human dental pulp cells, and that this is dependent on dynamin-related endocytosis and independent of protease activity. The objective of the present study was to determine the expression of CTGF/CCN2 in reparative dentin in human carious teeth and to examine the effect of CTGF/CCN2 on mineralization in cultured human dental pulp cells. Minimal expression of CTGF/CCN2 was evident in odontoblasts subjacent to the dentin-pulp junction in healthy teeth, whereas strong expression was detected in odontoblast-like cells lining the reparative dentin subjacent to dental caries. In human dental pulp cells, CTGF/CCN2 promoted mineralization but failed to induce proliferation, suggesting that this molecule has the ability to induce the differentiation of human dental pulp cells. Taken together, the data suggest that CTGF/CCN2 is likely involved in reparative dentinogenesis through formation of hard tissue in human carious teeth.